Gene Expression Property of High-Density Three-Dimensional Tissue of HepG2 Cells Formed in Radial-Flow Bioreactor(BIOINFORMATICS)

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概要

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• In our previous study, we examined three-dimensional culture using 5-ml radial-flow bioreactor (RFB) and showed that genes encoding cell cycle related proteins were suppressed in a stable phase. In this study, we analyzed the gene expression profiles of RFB-cultivated HepG2 cells and found that vascular endothelial growth factor (VEGF) production was strongly induced in the stable phase compared with the growth phase or static two-dimensional culture. When human umbilical vein endothelial cells (HUVECs) were grown under the conditioned medium of the stable phase, it was found that the formation of new blood vessels was induced in the angiogenesis model. DNA microarray analysis showed that the expression levels of both genes related to cell cycle arrest and which are known as tumor markers have increased in the stable phase. This result suggests that HepG2 cells in the stable phase maintain an active tumor phenotype. In addition, the expression of genes induced in the hypoxic condition was also induced in the stable phase. When the culture was carried out under a higher dissolved oxygen (DO) concentration, VEGF production did not decrease significantly and the new blood-vessel-forming ability of the conditioned medium was not suppressed. This suggests that the induction of VEGF production in a stable phase is not affected by DO during the tested level. These results suggest that the RFB cell culture system may be used to assess tumor progression mechanism under three-dimensional condition in vitro.

• 社団法人日本生物工学会の論文
• 2006-03-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• OZAWA Shogo

  Project Team for Pharmacogenetics, National Institute of Health Sciences

• Kawamoto Manabu

  Institute Of Rheumatology Tokyo Women's Medical University

• Sawada Junichi

  Project Team For Pharmacogenetics National Institute Of Health Sciences

• Ozawa Shogo

  Project Team For Pharmacogenetics National Institute Of Health Sciences

• OHNO Yasuo

  Division of Pharmacology, National Institute of Health Science

• Kitakaze Masafumi

  国立循環器病センター 心臓血管

• Kitakaze Masafumi

  Nagoya University Graduate School Of Medicine

• Tomoike Hitonobu

  国立循環器病センター 疫学部

• Tomoike Hitonobu

  国立循環器病センター研究所 疫学部

• Ohe Yuichiro

  国立医薬品食品衛生研究所

• Ohe Yuichiro

  Thoracic Oncology Division National Cancer Center Hospital National Cancer Center

• Ohe Yuichiro

  Department Of Medical Oncology National Cancer Center Hospital

• Ohe Yuichiro

  Department Of Internal Medicine National Cancer Center Hospital

• Ohno Yasuo

  Division Of Pharmacology National Institute Of Health Sciences

• OZAWA Shogo

  Division of Pharmacology, National Institute of Health Sciences

• Muro K

  国立がんセンター中央病院

• Muro Kei

  Gastrointestinal Oncology Division National Cancer Center Hospital

• Manabe Kasumi

  鳥取大学 医学系研究科再生医療学

• Miyatake Kunio

  国立病院機構大阪南医療センター 循環器科

• MINATOYA Kenji

  National Cardiovascular Center

• Ozawa S

  Project Team For Pharmacogenetics National Institute Of Health Sciences

• Matsuura Kaoru

  国立循環器病センター

• Sawada Jun-ichi

  Division Of Functional Biochemistry And Genomics National Institute Of Health Sciences

• Tomoike H

  Division Of Preventive Cardiology National Cardiovascular Center

• Ozawa Shogo

  Division Of Pharmacology National Institute Of Health Sciences

• Awata Masaki

  関西ろうさい病院 内科･循環器科

• Ishikawa Yoichi

  エイブル

• Hongo Tomokatsu

  ABLE Corporation

• Kajikawa Mariko

  ABLE Corporation

• Ishida Seiichi

  Division of Pharmacology, National Institute of Health Sciences

• Ishikawa Yoichi

  ABLE Corporation

• Kitakaze Masafumi

  Department Of Cardiovascular Medicine National Cardiovascular Center

• Minatoya Kenji

  Department Of Cardiovascular Surgery National Cardiovascular Center

• Sawada Jun-ichi

  Division Of Biochemistry And Immunochemistry Natinal Institute Of Health Sciences

• Ohno Y

  Division Of Pharmacology National Institute Of Health Sciences

• Ohno Y

  Natl. Inst. Health Sci.

• Ohno Yasuo

  Div. Of Pharmacology Biological Safty Research Center National Institute Of Hygienic Sciences

• Ohe Yuichiro

  Division Of Internal Medicine And Thoracic Oncology National Cancer Center Hospital

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Honda Hiroyuki

  Department Of Biotechnology School Of Engineering Nagoya University

• Ozawa Shogo

  National Institute Of Health Sciences

• Honda Hiroyuki

  Department Of Cardiovascular Research R.i.e.m. Nagoya University

• Ishida Seiichi

  Division Of Pharmacology National Institute Of Health Sciences

• Ishida Seiichi

  National Institute Of Health Sciences

• Manabe Kasumi

  Division Of Cardiovascular Medicine National Cardiovascular Center

• Tomoike Hitonibu

  Department Of Cardiology National Cardiovascular Center

• Ozawa Shogo

  Project Team For Pharmacogenetics National Inst. Of Health Sciences

• Tomoike Hitonobu

  Cardiology And Emergency Medicine National Cardiovascular Center

• Ishikawa Yoichi

  Able Co. Ltd.

• Sawada J

  Mie Univ. School Of Medicine Mie Jpn

• Okada Shuichi

  Hepatobiliary And Pancreatic Oncology Division National Cancer Center Hospital

• Omori Yoshihito

  Division Of Pharmacology National Institute Of Hygienic Sciences

• Omori Yoshihito

  Division Of Pharmacology Biological Safety Research Center National Institute Of Hygienic Sciences:d

• Ohno Yasuo

  National Inst. Of Health Sci. Tokyo Jpn

• Ohno Yasuo

  Division Of Internal Medicine And Thoracic Oncology National Cancer Center Hospital

• Ohkawa Shinichi

  Division Of Hepatobiliary And Pancreatic Oncology Kanagawa Cancer Center Hospital

• Sawada J

  National Inst. Of Health Sci. Tokyo Jpn

• Okamoto Yuko

  Department Of Internal Medicine National Cancer Center Hospital

• Ohe Y

  National Cancer Center Hospital Tokyo

• Kitakaze Masafumi

  First Department Of Medicine And Department Of Pathophysiology Osaka University School Of Medicine

• Kadoma Masaaki

  First Department Of Medicine And Department Of Pathophysiology Osaka University School Of Medicine

• Takeda Hideo

  Department Of Preventive Medicine Institute Of Health Biosciences The University Of Tokushima Gradua

• OHKAWA Shinichi

  Kanagawa Cancer Center Hospital

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Kadoma Masaaki

  The First Department Of Medicine Osaka University School Of Medicine

• Ozawa Shogo

  Department Of Pharmacodynamics And Molecular Genetics School Of Pharmacy Iwate Medical University

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