Human Antithrombin III variant Production from Recombinant BHK Cells in a Fed-Batch Culture with On-Line Control of Glucose and Glutamine Concentrations

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概要

• 論文の詳細を見る

• Human antithrombin III variant(AT III)production from recombinant BHK cells was investigated in a spinner flask fed-batch culture associated with an on-line HPLC system. Six experiments were carried out using three set points for the glucose concentration(0.5, 2.0, and 3.0g/l)and two set points for the glutamine concentration(0.2 and 0.6g/l). The highest concentration of AT III, which was obtained in the experiment with 3.0g/l glucose and 0.2g/l glutamine, was about two times higher than the lowest concentration obtained. The monosaccharide content of the AT III produced was almost the same in all the experiments, though the amount of sialic acid detected in the AT III produced in two of the experiments with 3.0g/l of glucose was a little on the low side.

• 社団法人日本生物工学会の論文
• 1998-05-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• KITADE Tamotsu

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Kitade Tamotsu

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Lenas Petros

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• PETROS LENAS

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• WATANABE HIDEKI

  Tsukuba Research Laboratories, Eisai Co. Ltd.

• Watanabe Hideki

  Tsukuba Research Laboratories Eisai Co. Ltd.

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

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