Development of Rotating-Mesh Basket Type Bioreactor for Carrot Embryo Production in Immobilized Callus System

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概要

• 論文の詳細を見る

• Mass-production of regenerated plantlets using alginate bead encapsulating carrot callus was studied. When gel beads were cultivated in a flask containing regeneration medium, somatic embryos were released at high frequency from the gel beads and developed into plantlets. When a callus less than 320 μm in size was immobilized in the beads, a larger number of embryo was released from the beads. At 0.11 ml-pcv/ml-gel of inoculum size, the maximum number of embryo was obtained. In order to develop a suitable bioreactor for the immobilized callus system, three types of bioreactors, namely air-lift, horizontally shaking vessel and rotating mesh-basket types, were tested. In the case of the rotating mesh-basket type, a larger amount of released cells was obtained. Plantlet formation from the released cells reached a level of 115% compared with that of the flask culture. To achieve continuous recovery of released cells and perform long term culture, a cyclone type cell separator was designed and used in conjunction with a rotating mesh-basket type bioreactor. When 110 ml/min of medium flow was passed through the separator, 90% of suspended cells were collected after 40 min of operation time. Long term cultures at 50, 100, and 150 rpm of rotational speed were carried out. In the experiment at 100 rpm, almost constant values of released cell volume and number of embryos were obtained during 12 d. They were 3 ml-pcv/l-medium/d and 7000 plantlets/g-released cells, respectively.

• 社団法人 化学工学会の論文

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• Nagamori Eiji

  Department Of Biotechnology School Of Engineering Nagoya University

• Nagamori Eiji

  Toyota Central R & D Labs. Inc.

• Nagamori E

  Osaka Univ. Osaka Jpn

• Nagamori Eiji

  Biotechnology Laboratory Toyota Central R&d Labs Inc.

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Nagamori Eiji

  Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Uozumi N

  Nagoya Univ. Nagoya Jpn

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• UOZUMI NOBUYUKI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• SUEHARA Ken-ichiro

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Suehara Ken-ichiro

  Hiroshima City University

• Uozumi Nobuyuki

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Nagamori Eiji

  Department Of Biotechnology Graduate School Of Engineering Osaka University

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