Effective tPA Production by BHK Cells in Nutrients-Controlled Culture Using an On-Line HPLC Measuring System

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概要

• 論文の詳細を見る

• A baby hamster kidney (BHK) cell line producing recombinant tissue plasminogen activator (tPA) was cultured with control of both the glucose and glutamine concentrations by an on-line HPLC measuring and control system. It was found that BHK cell growth showed more significant responses to glutamine than to glucose. An approximately 2-fold increase in the viable cell concentration was observed when glutamine was controlled at the low levels of 0.1 and 0.5 g/l compared to that when it was controlled at 1.0 g/l. Maximum tPA production was obtained in a control culture with 1.0 g/l-glucose and 0.5 g/l-glutamine. Accumulation of lactate and ammonia showed respectively less dependence on the glucose and glutamine concentrations in the medium.

• 社団法人日本生物工学会の論文
• 1995-07-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• WANG Jin

  Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Wang J

  Department Of Physics The University Of Hong Kong

• Wang Jin

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Wang Jin

  Laboratory Of Analytical Microbiology National Center For Biomedicine Analysis Institute Of Microbio

• Lenas Petros

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• WATANABE HIDEKI

  Eisai Co. Ltd., Tsukuba Research Laboratories

• Watanabe Hideki

  Tsukuba Research Laboratories Eisai Co. Ltd.

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Wang Jin

  Department Of Biological Sience And The Mervin Bovaird Center For Studies In Molecular Biology And B

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