Release of Embryogenic Carrot Cells with High Regeneration Protency from Immobilized Alginate Beads

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概要

• 論文の詳細を見る

• For the mass-production of regenerated carrot plantlets, embryogenic carrot callus immobilized in calcium alginate gel beads was cultivated in a growth medium and the regeneration frequency of cells released from alginate gel beads was compared with that in a suspension culture. Cells released in the immobilized culture were regenerated at a frequency which was about 1.5 times higher than that obtained in the suspension culture. When CaCl_2 was added to the growth medium at 5 mM, repeated batch culture for plantlet production continued for 245d with no significant decrease in the productivity(1.6×10^5 plantlets/l-medium/d).

• 社団法人日本生物工学会の論文
• 1999-08-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• Nagamori Eiji

  Department Of Biotechnology School Of Engineering Nagoya University

• Nagamori Eiji

  Toyota Central R & D Labs. Inc.

• Nagamori E

  Osaka Univ. Osaka Jpn

• Nagamori Eiji

  Biotechnology Laboratory Toyota Central R&d Labs Inc.

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Nagamori Eiji

  Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Nagamori Eiji

  Department Of Biotechnology Graduate School Of Engineering Osaka University

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