Development of Chitosan-Magnetite Aggregates Containing Nitrosomonas europaea Cells for Nitrification Enhancement

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概要

• 論文の詳細を見る

• A cell suspension of the nitrifying bacterium Nitrosomonas europaea obtained after 96-h cultivation was subjected to mangetic separation using chitosan-conjugated magnetite particles(chitosan-magnetite), which have the ability to form aggregates with microbial cells. An equilibrium condition was obtained at room temperature after 30 min and over 90% of the cells were recovered when the chitosan-magnetite concentration was 200mg/l. The relationship between the cell concentration in the supernatant in equilibrium and the number of cells adsorbed per 1g chitosan-magnetite was expressed by a Freundlich-type adsorption equation. A high nitrifying bacterium activity yield was obtained with a chitosan-magnetite concentration between 100 and 200mg/l. Repeated batch culture resulted in more N.europaea cells accumulating on the aggregates and as a consequence their nitrification activity improved further. The chitosan-magnetite/cell aggregates were recovered and employed to remove ammonia from artificial wastewater together with PVA-alginate gel beads containing the denitrifying bacterium Paracoccus denitrificans. A higher ammonia removal rate was achieved under aerobic conditions in comparison with that obtained when N.europaea and P.denitrificans were coimmobilized in PVA-alginate gel beads.

• 社団法人日本生物工学会の論文
• 2000-05-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• OHSHIMA AKIRA

  Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• SHINKAI Masashige

  Department of Biotechnology, Graduate School of Engineering, Nagoya university

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Ohshima Akira

  Department Of Biotechnology Graduate School Of Engoneering Nagoya University

• LIU CHUNZHAO

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Shinkai Masashige

  Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn

• Liu Chunzhao

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Shinkai Masashige

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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