Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays

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概要

• 論文の詳細を見る

• To investigate the biological mechanism of organic solvent tolerance (OST), DNA microarrays were used to collect and compare the gene expression profiles of normal and organic solvent-toler-ant Escherichia coli strains. First, we compared the tolerant-strain OST3410 to its sensitive parent strain JA300 in the absence of organic solvents. Numerous genes showed higher expression levels in OST3410, and Northern analysis was used to confirm the higher expression level of some genes. Next, the gene expression profiles of JA300 and OST3410 exposed to hexane as an organic solvent were investigated and compared with JA300 before exposure to organic solvent. In OST3410 and JA300, 115 and 47 hexane-induced genes were found, respectively. As candidates for genes related to OST, we focused on six genes : cysD, marA, mglB, tnaA, tnaB and yihM, which were upregulated by hexane in both strains. When these genes were over-expressed on plasmids, only the marA plasmid increased OST activity. It should be noted that we succeeded in finding a gene related to OST activity using only DNA microarray data, without any biochemical or biological knowledge.

• 社団法人日本生物工学会の論文
• 2003-04-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• 本多 裕之

  名大院工・生物機能

• Kobayashi T

  School Of Bioscience And Biotechnology Chubu University

• 花井 泰三

  Department Of Biotechnology School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kobayasi T

  School Of Bioscience And Biotechnology Chubu University

• HANAI Taizo

  Department of Biochemistry and Engineering, Tohoku University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• HAYASHI SHUHEI

  Department of Pediatrics, Fukui Red Cross Hospital

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Hanai Taizo

  Department Of Biochemistry And Engineering Tohoku University

• AONO Rikizo

  Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology

• MORI HIROTADA

  Research and Education Center for Genetic Information,Nara Institute of Science and Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Aono Rikizo

  Department Of Biological Information School Of Bioscience And Biotechnology Tokyo Institute Of Techn

• Mori Hirotada

  Research And Education Center For Genetic Information Nara Institute Of Science And Technology

• Hayashi S

  Department Of Biotechnology School Of Engineering Nagoya University

• Hayashi Shuhei

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology

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