OVERPRODUCTION OF FOREIGN GENE PRODUCT IN RECOMBINANT ESCHERICHIA COLI BY IN SITU ADSORPTION OF TRYPTOPHAN
スポンサーリンク
概要
- 論文の詳細を見る
For two stage culture with control of tryptophan concentration, rapid in situ removal of tryptophan by adding adsorbent was investigated. Activated carbon, BAC, could adsorb a larger amount of tryptophan than other adsorbents. From test tube cultures, it was found that 20 g/1 of BAC was necessary to remove large portions of tryptophan in a medium containing 20 mg/1 of tryptophan and induce the β-galactosidase gene expression. Two stage culture was performed using a jar fermentor in which tryptophan concentration was kept at 20 mg/l in the first stage for cell growth and then almost totally removed by direct addition of 20 g/l of BAC to the medium. One hour after addition of BAC, an increase in enzyme activity was observed. Maximum specific enzyme activity reached a final value of 320 μkat/g-protein, which corresponded to a level 12 times that of the control culture.
- 社団法人 化学工学会の論文
- 1994-10-20
著者
-
Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
-
KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
-
Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
-
Honda Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University
-
KOBAYASHI TAKESHI
(Present address) Department of Chemical Engineering, Faculty of Engineering, Nagoya University
-
OGISO Koji
Department of Biotechnology, Faculty of Engineering, Nagoya University
-
ARIGA OSAMU
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
-
YAMAKAWA TATEKI
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
-
SANO YOSHIKI
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
-
Ariga O
Shinshu Univ. Nagano Jpn
-
Ariga Osamu
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
-
Sano Y
Shinshu Univ. Nagano Jpn
-
Sano Y
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
-
Yamakawa Tateki
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
-
Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
-
Sano Yoshiki
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
-
Kobayashi T
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
-
Yamakawa T
Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo
-
Kobayashi T
Univ. Tsukuba Ibaraki Jpn
-
Kobayashi Takeshi
Dept.of Chemical Engineering Nagoya University
-
Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
-
Kobayashi Takeshi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
-
Kobayashi Takeshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
-
Kobayashi Takeshi
Department Of Biotechnology:faculty Of Engineering Nagoya University
-
KOBAYASHI TAKESHI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
-
KOBAYASHI TAKESHI
Department of Biotechnology, Faculty of Engineering, Nagoya University
-
ARIGA OSAMU
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
関連論文
- Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system(METHODS)
- Scale-up fermentation of echinocandin type antibiotic FR901379(BIOCHEMICAL ENGINEERING)
- Improvement of FR901379 production by mutant selection and medium optimization(BIOCHEMICAL ENGINEERING)
- Directed evolution of angiotensin II-inhibiting peptides using a microbead display(METHODS)
- Magnetic manipulation device for the optimization of cell processing conditions(MEDICAL BIOTECHNOLOGY)
- Practical cell labeling with magnetite cationic liposomes for cell manipulation(METHODS)
- Effect of Global Transcriptional Regulators Related to Carbohydrate Metabolism on Organic Solvent Tolerance in Escherichia coli(BIOCHEMICAL ENGINEERING)
- Production of Recombinant Chicken IgY-Fc and Evaluation of Its Transport Ability into Avian Egg Yolks
- Directed evolution of angiotensin II-inhibiting peptides using a microbead display
- AN-P24 Magnetic manipulation device for the optimization of cell processing(Section I Animal and Plant Cell Cultures)
- Screening of Epithelial Cell-Adhesive Peptides from Fibronectin Loop Region and Its Cell Specificity
- A Motif Detection and Classification Method for Peptide Sequences Using Genetic Programming(BIOINFORMATICS)
- Mag-Seeding of Rat Bone Marrow Stromal Cells into Porous Hydroxyapatite Scaffolds for Bone Tissue Engineering(CELL AND TISSUE ENGINEERING)
- Effective Cell-Seeding Technique Using Magnetite Nanoparticles and Magnetic Force onto Decellularized Blood Vessels for Vascular Tissue Engineering(CELL AND TISSUE ENGINEERING)
- Gene Expression Property of High-Density Three-Dimensional Tissue of HepG2 Cells Formed in Radial-Flow Bioreactor(BIOINFORMATICS)
- Three-Dimensional High-Density Culture of HepG2 Cells in a 5-ml Radial-Flow Bioreactor for Construction of Artificial Liver(Biochemical Engineering)
- Targeting Hyperthermia for Renal Cell Carcinoma Using Human MN Antigenspecific Magnetoliposomes
- Cancer DNA Microarray Analysis Considering Multi-subclass with Graph-based Clustering Method(BIOINFORMATICS)
- Identification of HLA-A24-Restricted Epitopes with High Affinities to Hsp70 Using Peptide Arrays(METHODS)
- Screening of Cell-Adhesive Peptide from the Human Laminin-5 α3 Chain Globular 2 and 3 Domains
- Screening of Anti-Tumor Peptide Vaccines with High Affinity to Hsp70 Using Peptide Array
- Peptide Informatics - Novel Method for Screening and Design of Biologically Active Peptides
- Peptide Array-Based Interaction Assay of Solid-Bound Peptides and Anchorage-Dependant Cells and Its Effectiveness in Cell-Adhesive Peptide Design (BIOINFORMATICS)
- Hidden Markov Model-Based Prediction of Antigenic Peptides That Interact with MHC Class II Molecules
- Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Prediction of Peptide Binding to Major Histocompatibility Complex Class II Molecules through Use of Boosted Fuzzy Classifier with SWEEP Operator Method(BIOINFORMATICS)
- Expression of a Thermostable Cellulase Gene from a Thermophilic Anaerobe in Saccharomyces cerevisiae
- Production of Ethanol in Repeated-Batch Fermentation with Membrane-Type Bioreactor
- Diversity of Cellulases Produced by a Thermophilic Anaerobe Strain NA10
- Molecular Cloning of Ruminococcus albus Cellulase Gene(Biological Chemistry)
- Incorporation of Capillary-Like Structures into Dermal Cell Sheets Constructed by Magnetic Force-Based Tissue Engineering
- Application of Ultra-Water-Repellent Surface to Cell Culture(CELL AND TISSUE ENGINEERING)
- Heat Immunotherapy Using Magnetic Nanoparticles and Dendritic Cells for T-Lymphoma(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
- Screening of Cytokines to Enhance Vaccine Effects of Heat Shock Protein 70-Rich Tumor Cell Lysate(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
- Medical Application of Functionalized Magnetic Nanoparticles(Advances in Biomedical Science and Engineering)
- Complete Regression of Mouse Mammary Carcinoma with a Size Greater than 15 mm by Frequent Repeated Hyperthermia Using Magnetite Nanoparticles
- Selective hyperthermia using magnetoliposomes to target cervical lymph node metastasis in a rabbit tongue tumor model
- Proliferation and Stratification of Keratinocyte on Cultured Amniotic Epithelial Cells for Tissue Engineering(MEDICAL BIOTECHNOLOGY)
- Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma
- Transglutaminase-Mediated Gelatin Matrices Incorporating Cell Adhesion Factors as a Biomaterial for Tissue Engineering
- Radiation-Inducible TNF-α Gene Expression under Stress-Inducible Promoter gadd 153 for Cancer Therapy
- Bystander-Killing Effect and Cyclic Induction of TNF-α Gene under Heat-Inducible Promoter gadd 153
- Colominic Acid Production from Escherichia coli in a Fed-Batch Culture under the Control of Ammonium Ions Using an FIA System
- Effective Lactic Acid Production by Two-Stage Extractive Fermentation
- Time-Course Data Analysis of Gene Expression Profiles Reveals purR Regulon Concerns in Organic Solvent Tolerance in Escherichia coli(Microbial Physiology and Biotechnology)
- SELECTION CRITERIA OF OXYGEN CARRIERS UTILIZABLE FOR AUTOTROPHIC GROWTH IMPROVEMENT OF ALCALIGENES EUTROPHUS UNDER LOWERED OXYGEN PARTIAL PRESSURE
- ANALYSIS OF OXYGEN TRANSFER ENHANCEMENT BY OXYGEN CARRIER IN THE AUTOTROPHIC CULTIVATION OF ALCALIGENES EUTROPHUS UNDER LOW OXYGEN PARTIAL PRESSURE
- PHYTOHORMONE CONTROL FOR PLANT CELL CULTURE USING A BIOREACTOR EQUIPPED WITH IN-LINE FEEDING COLUMN
- A MODEL ANALYSIS OF MICROBIAL RETAINMENT PROCESS IN POROUS SUPPORT PARTICLES IN A FLUIDIZED-BED WASTEWATER TREATMENT REACTOR
- Preparation of L929 Cell Array by Magnetic Pin Holder Device for Single Cell Function Analysis
- OVERPRODUCTION OF FOREIGN GENE PRODUCT IN RECOMBINANT ESCHERICHIA COLI BY IN SITU ADSORPTION OF TRYPTOPHAN
- 4-S-Cysteaminylphenol-loaded magnetite cationic liposomes for combination therapy of hyperthermia with chemotherapy against malignant melanoma
- Prognostic models in patients with non-small-cell lung cancer using artificial neural networks in comparison with logistic regression
- Proposal of Pumpless, Valveless, and Flowless Miniaturized Reactor Using Magnetic Beads for the Portable Analysis Device
- Fabrication of Antigen Sensors Using Carbon Nanotube Field Effect Transistors
- Preparation of Tumor-Specific Magnetoliposomes and Their Application for Hyperthermia
- Fabrication of vertically-aligned CNT electrodes using plasma-enhanced CVD for chemical sensors
- Modeling of Consumers' Preferences for Regular Coffee Samples and Its Application to Product Design
- Determination of the Blending Ratio of Regular Coffee Samples by Information Technology
- Multiple fuzzy neural network system for outcome prediction and classification of 220 lymphoma patients on the basis of molecular profiling
- Selection of Causal Gene Sets for Lymphoma Prognostication from Expression Profiling and Construction of Prognostic Fuzzy Neural Network Models(MEDICAL BIOTECHNOLOGY)
- Fuzzy Neural Network Applied to Gene Expression Profiling for Predicting the Prognosis of Diffuse Large B-cell Lymphoma
- Inference of Common Genetic Network Using Fuzzy Adaptive Resonance Theory Associated Matrix Method(BIOINFORMATICS)
- Artificial Neural Network Predictive Model for Allergic Disease Using Single Nucleotide Polymorphisms Data
- Determination of Operating Conditions in Activated Sludge Process Using Fuzzy Neural Network and Genetic Algorithm
- Construction of COD Simulation Model for Activated Sludge Process by Recursive Fuzzy Neural Network
- Construction of COD Simulation Model for-Acivated Sludge Process by Fuzzy Neural Network
- Magnetic Characterization of Magnetite Particles for MR Contrast Agents
- A Preprocessing Method for Inferring Genetic Interaction from Gene Expression Data Using Boolean Algorithm(BIOINFORMATICS)
- Prognostic Predictor with Multiple Fuzzy Neural Models Using Expression Profiles from DNA Microarray for Metastases of Breast Cancer(BIOINFORMATICS)
- Proposal of Reliability Index in Search for Reliable Solutions of Reverse Calculation Based on Fuzzy Neural Network Modeling
- Evaluation of the Alzheimer-Type Dementia by Magnetic Resonance Imaging Using Fuzzy Neural Networks
- Simple and Rapid Cell Growth Assay Using Tetrazolium Violet Coloring Method for Screening of Organic Solvent Tolerant Bacteria
- Construction of an Electroencephalogram-Based Brain-Computer Interface Using an Artificial Neural Network(Welfare Engineering)
- Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays
- A New Reliable Cancer Diagnosis Method Using Boosted Fuzzy Classifier with a SWEEP Operator Method
- Association Study between Apolipoprotein L and Schizophrenia by Exhaustive and Rule-Based Combination Analysis for Identification of Multilocus Interactions(BIOINFORMATICS)
- Proposal of New Gene Filtering Method, BagPART, for Gene Expression Analysis with Small Sample(BIOINFORMATICS)
- Classification of Intramural Metastases and Lymph Node Metastases of Esophageal Cancer from Gene Expression Based on Boosting and Projective Adaptive Resonance Theory(BIOINFORMATICS)
- Classification Method for Predicting the Development of Myocardial Infarction by Using the Interaction between Genetic and Environmental Factors
- Prediction of Occurrence of Heterocapsa circularisquama Red Tide by Means of Fuzzy Neural Network
- Human Antithrombin III variant Production from Recombinant BHK Cells in a Fed-Batch Culture with On-Line Control of Glucose and Glutamine Concentrations
- Performance of a Shaking Vessel-Type Bioreactor with a Current Pole
- Lymphoma Prognostication from Expression Profiling Using a Combination Method of Boosting and Projective Adaptive Resonance Theory
- Development of Rotating-Mesh Basket Type Bioreactor for Carrot Embryo Production in Immobilized Callus System
- Characterization on Heat Sterilization of a Novel Autoclave with a Turntable for Rotation
- Fuzzy Neural Network-Based Prediction of the Motif for MHC Class II Binding Peptides
- Enhanced Anthocyanin Production from Grape Callus in an Air-Lift Type Bioreactor Using a Viscous Additive-Supplemented Medium
- Enhanced and Prolonged Production of Plantlets Regenerated from Carrot Callus in a Viscous Additive-Supplemented Medium
- Lipase Production in Two-Step Fed-Batch Culture of Organic Solvent-Tolerant Pseudomonas aeruginosa LST-03
- Release of Embryogenic Carrot Cells with High Regeneration Protency from Immobilized Alginate Beads
- Prolonged loss of leg myogenic motor evoked potentials during thoracoabdominal aortic aneurysm repair, without postoperative paraplegia
- Development of Chitosan-Magnetite Aggregates Containing Nitrosomonas europaea Cells for Nitrification Enhancement
- PRODUCTION OF Fv FRAGMENT OF MONOCLONAL ANTIBODY FROM RECOMBINANT METHYLOTROPHIC YEAST, PICHIA PASTORIS
- Effective tPA Production by BHK Cells in Nutrients-Controlled Culture Using an On-Line HPLC Measuring System
- Enhancement of tPA Production under Hyperoxic Conditions by BHK Cells in Serum-Free and Serum-Containing Cultures
- Rapid Enzyme-Linked Immunosorbent Assay with Functional Magnetite Particles
- Development of Chitosan-Conjugated Magnetite for Magnetic Cell Separation
- Purification and Characterization of Recombinant Bovine Cathepsin C
- Effcient Production of Recombinant Bovine Cathepsin C by High Cell Density Culture of Methylotrophic Yeast Candida boidinii