Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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In a previous study, we reported the effectiveness of a bacterial strain showing monolayer adsorption to oil surfaces on microbial conversion at oil-water interfaces. In the present study, we screened wild type strains from our toluene-degrading bacterial library that showed similar properties and succeeded in obtaining five wild type strains that adsorb to oil surfaces as a cell monolayer. We investigated the effects of cultivation conditions on cell surface hydrophobicity of these five strains. The effects of substrate hydrophobicity and the porous carrier were not significant. By contrast, growth temperature greatly affected the cell surface hydrophobicity of all five strains, especially strain TIS1-127, which was phylogenetically identified as Pseudomonas sp. which is closely related to P. mosselii, P. monteilii, and P. plecoglossicida. Pseudomonas sp. TIS1-127 cells grown at 37℃ were determined by the kinetic microbial-adhesion-to-hydrocarbon(MATH) test to be fully hydrophilic(lower than 10% of MATH value) while the cells grown at 28℃ were highly hydrophobic(over 90% of MATH value). We investigated the effects of growth temperature on toluene conversion by TIS1-127 resting cells in single-phase batch cultivation and in two-liquid-phase partitioning reactors containing an emulsion consisting of 20% silicone oil and 80% cell suspension. In both cases, the cells grown at 28℃ showed much higher conversion ability than those grown at 37℃. Toluene conversion followed Michaelis-Menten kinetics and the K_m values for the cells grown at 28℃ were lower than 1/10 those for the cells grown at 37℃.
- 社団法人日本生物工学会の論文
著者
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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本多 裕之
名大院・工・生物機能
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Okochi Mina
Department of Biotechnology, School of Engineering, Nagoya University
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Kanie Kei
Department of Biotechnology, School of Engineering, Nagoya University
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Honda Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc
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Watanabe Hisami
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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Hori Katsutoshi
Department of Materials Science and Engineering, Nagoya Institute of Technology
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Kanie Kei
Department Of Biotechnology School Of Engineering Nagoya University
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Hiramatsu Naoto
Department of Materials Science and Engineering, Nagoya Institute of Technology
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Nannbu Mari
Department of Materials Science and Engineering, Nagoya Institute of Technology
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Honda Hiroyuki
Department Of Biotechnology School Of Engineering Nagoya University
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Okochi Mina
Department Of Biotechnology School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Cardiovascular Research R.i.e.m. Nagoya University
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Hori Katsutoshi
Dep. Of Materials Sci. And Engineering Nagoya Inst. Of Technol. Gokiso-cho Showa-ku Nagoya 466-8555
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Hori Katsutoshi
Department Of Materials Science And Engineering Nagoya Institute Of Technology:project Research Cent
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Watanabe Hisami
Graduate School Of Bioscience And Biotechnology Tokyo Inst. Of Technol. Yokohama 226-8501 Jpn
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Watanabe Hisami
Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Honda Hiroyuki
Graduate School Of Engineering Nagoya University
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Hori Katsutoshi
Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology:department Of Material
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Hosokawa Hiroyuki
Department Of Immunology Graduate School Of Medicine Chiba University
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Honda Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Hiramatsu Naoto
Department Of Materials Science And Engineering Nagoya Institute Of Technology
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Nannbu Mari
Department Of Materials Science And Engineering Nagoya Institute Of Technology
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Hamada Hirobumi
Department Of Molecular Medicine Sapporo Medical University School Of Medicine
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Okochi Mina
Department Of Biotechnology Nagoya University
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Hamada Hirobumi
Department Of Biotechnology School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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Hori Katsutoshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Okochi Mina
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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