Effective Lactic Acid Production by Two-Stage Extractive Fermentation

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概要

• 論文の詳細を見る

• For effective microbial lactic acid production using Lactobacillus delbrueckii, two-stage extractive fermentation was examined. Extractants were screened from the viewpoints of a high distribution coefficient for lactic acid and less toxicity toward the microorganism. Even if the extractant showed some toxicity toward the microorganism, it was found that a reduction of toxicity was possible by back-extraction using oleyl alcohol. As a result, 40% Alamine 336 diluted with oleyl alcohol, and oleyl alcohol, were selected as the extractant and the back-extractant, respectively. After two-stage extraction by these extractants, the growth rate was improved by the removal of lactic acid. This method was then applied to continuous extractive fermentation using a jar-fermentor. During 4-h extraction, lactic acid accumulation in the broth was significantly suppressed, while the cell growth and glucose consumption rates were also found not to be reduced. After 24 h, the cell concentration attained an OD _<660> of 14,which corresponded to a level 1.25 times higher than that of the control culture without extraction. Total lactic acid productivity was 1.4 times level compared with the control culture.

• 社団法人日本生物工学会の論文
• 1995-06-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Honda Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  (Present address) Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• NAKAZEKO TAKUO

  Department of Biotechnology, Garaduate School of Engineering, Nagoya University

• TOYAMA YOSHIO

  Nagatanien Co.,

• TAKAHASHI HIROSHI

  Nagatanien Co.,

• Toyama Yoshio

  Nagatanien Co.

• Nakazeko Takuo

  Department Of Biotechnology Garaduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Kobayashi Takeshi

  Dept.of Chemical Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Kobayashi Takeshi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology:faculty Of Engineering Nagoya University

• Takahashi Hiroshi

  Nagatanien Co.

• KOBAYASHI TAKESHI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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