Rapid Selection of Nonhotspot Mutants among hisD^+ Revertants of Salmonella typhimurium TA98 in Ames Test by Peptide Nucleic Acid (PNA)-Mediated PCR Clamping(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Ames test is the most popular method of assessing mutagenicity using Salmonella typhimurium as an indicator. Recently, sequence analyses have been introduced for the investigation of mutation mechanisms. Most revertants (>70%) carry 2-bp deletion within an 8-bp CG repeat in hisD (hotspot mutation) in the Ames test using S. typhimurium TA98. We developed a new specific amplification method for nonhotspot mutants by peptide nucleic acid (PNA)-mediated PCR clamping. It markedly reduces the labor and cost of this kind of studies.
- 社団法人日本生物工学会の論文
- 2003-12-25
著者
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KAWAI Keiichi
Faculty of Health Science, School of Medicine, Kanazawa University
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TAKIYA Toshiyuki
United Graduate School of Agricultural Science, Gifu University
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FUTO Satoshi
Nippon Flour Mills Co., Ltd.
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Kawai K
Faculty Of Applied Biological Sciences Gifu University
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Futo Satoshi
Nippon Flour Mills Co. Ltd.
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Futo Satoshi
Fasmac Co. Ltd.
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Suzuki Tohru
Life Science Research Center, Gifu University
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Matsumoto Yutaka
Hokkaido Institute of Environmental Science
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Takiya Toshiyuki
United Graduate School Of Agricultural Science Gifu University
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Suzuki T
Ihara & Company Ltd.
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Suzuki Tohru
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Kawai Keiichi
Faculty Of Applied Biological Sciences Gifu University
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Kawai K
Fac. Appl. Biol. Sci. Gifu Univ.
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Kawai Keiichi
School Of Health Sciences Faculty Of Medicine Kanazawa University
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HORIE YOSHIAKI
Faculty of Agriculture, Gifu University
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Horie Yoshiaki
Faculty Of Agriculture Gifu University
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Kawai Keiichi
Fac. Appl. Biol. Sci. Gifu Univ.
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Matsumoto Yutaka
Hokkaido Research Institute For Environmental Sciences
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Suzuki Tohru
Life Science Research Center Gifu University
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Kamimura Kiyohisa
Department Of Radiology Fujimoto Hayasuzu Hospital
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