Immunoblotting Analysis of nsLTP1 in Cereal Grains with Antiserum Raised against Recombinant Rice nsLTP1
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概要
- 論文の詳細を見る
Nonspecific lipid transfer protein 1 (nsLTP1) is widely distributed in plants and has been recognized as a widely cross-reacting plant pan-allergen. In the present study, a recombinant protein of rice nsLTP1 was prepared with an Escherichia coli expression system, and polyclonal antiserum was raised by immunizing rabbits with this recombinant nsLTP1. The antiserum specifically immunoreacted with rice nsLTP1 and homologous nsLTP1 proteins of other kinds of cereal grains. Moreover, the antiserum was applied to compare nsLTP1 content among rice cultivars and among rice fractions separated by grain-milling. Variation of nsLTP1 contents were observed among rice cultivars, and rice nsLTP1 was proved to be mainly located in the outer layer of rice grains, providing evidence for decreasing nsLTP1 content by grain-milling.
- 社団法人 日本食品科学工学会の論文
- 2011-11-01
著者
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Kitta Kazumi
National Food Res. Inst.
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Kawamoto Shinichi
National Food Research Institute
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Kawamoto Shinichi
National Food Res. Inst.
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Ohnishi Kameyama
National Agriculture And Food Research Organization National Food Research Institute
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MORIYAMA Tatsuya
Department of Applied Cell Biology, Graduate School of Agriculture, Kinki University
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LANG Gang-hua
National Food Research Institute
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Moriyama Tatsuya
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
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KAGIYA Yukari
National Food Research Institute, National Agriculture and Food Research Organization
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Kawamoto Shinichi
National Food Research Institute National Agriculture And Food Research Organization
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Kagiya Yukari
National Food Research Institute National Agriculture And Food Research Organization
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Kitta Kazumi
National Food Research Institute, National Agriculture and Food Research Organization
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