Isolation and Characterization of Enterocin SE-K4 Produced by Thermophilic Enterococci, Enterococcus Faecalis K-4

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概要

• 論文の詳細を見る

• Enterococcus sp. K-4,with a bacteriocin-like activity against E. faecium, was isolated from grass silage in Thailand. Morphological, physiological, and phylogenetic studies clearly identified strain K-4 as a strain of E. faecalis. Strain K-4 produced a maximal amount of bacteriocin at 43-45℃. We purified, for the first time, the bacteriocin produced at high temperature by E. faecalis to homogeneity, using adsorption on cells of the producer strain and reversed-phase liquid chromatography. The bacteriocin, designated enterocin SEK4,is a peptide of about 5kDa as measured by SDSPAGE, and Mass spectrometry analysis found the molecular mass of 5356.2,which is in good agreement. The amino acid sequencing of the N-terminal end of enterocin SE-K4 showed apparent sequence similarity to class IIa bacteriocins. Enterocin SE-K4 was active against E. faecium, E. faecalis, Bacillus subtilis, Clostridium beijerinckii, and Listeria monocytogenes. Enterocin SE-K4 is very heat stable.

• 社団法人日本農芸化学会の論文
• 2001-02-23

著者

• Ogata Seiya

  Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University

• Ogata Seiya

  Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Eguchi Tadashi

  Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.

• Eguchi T

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Eguchi Tadashi

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Eguchi T

  Tokyo Inst. Technol. Tokyo

• Doi Katsumi

  Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University

• Shima Jun

  National Food Research Institute

• Eguchi T

  Kyushu Univ. Fukuoka Jpn

• Ohmomo Sadahiro

  Institute Of Applied Biochemistry University Of Tsukuba

• Ohmomo Sadahiro

  Institute Of Appried Biochemistry University Of Tsukuba ; (present Office)national Grassland Researc

• Ohmomo Sadahiro

  National Grassland Research Institute

• Ohmomo Sadahiro

  草地試験場

• Kawamoto Shinichi

  National Food Research Institute

• Choi Seong-hyun

  Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University

• EGUCHI Tomoko

  Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

• KAMINAKA Kazuyo

  National Food Research Institute

• MORI Katsumi

  National Food Research Institute

• CHOI Seong-Hyun

  Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

• DOI Katsumi

  Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

• Kawamoto Shinichi

  National Food Res. Inst.

• Shima J

  National Food Res. Inst. Ibaraki Jpn

• Eguchi T

  Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.

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