A Comparative Study and Phage Typing of Silage-Making Lactobacillus Bacteriophages
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概要
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To investigate basic characteristics of 10 virulent phages active on silage-making lactobacilli, morphological properties, host ranges, protein composition and genome characterization were separated into five groups based on host ranges and basic properties. The seven phages of groups I, II and V were active on Lactobacillus plantarum and Lactobacillus pentosus, Phage φPY4 (group III) infected both L. casei and Lactobacillus rhamnosus. Phage φPY5 (group IV) specifically infected Lactobacillus casei. Morphologically, three phages of groups I belonged to the Myoviridae family, while seven other phages of groups II, III and V belonged to the Siphoviridae family. SDS-PAGE profiles, restriction analysis, G+C contents of DNA and Dot blot hybridization revealed a high degree of homology in each group. Clustering derived from host range analysis was closely related to results of DNA and protein analyses. These phages may be applicable to phage typing for silage-making lactobacilli.
- 社団法人日本生物工学会の論文
- 2003-05-25
著者
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Ogata Seiya
Laboratory of Applied Microbiology, Faculty of Agriculture,Kyushu University
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Zhang Ye
Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University
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Ogata Seiya
Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University
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Doi Katsumi
Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University
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Ohmomo Sadahiro
Institute Of Applied Biochemistry University Of Tsukuba
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Ohmomo Sadahiro
Japan International Research Center For Agricultural Sciences
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Ohmomo Sadahiro
Institute Of Appried Biochemistry University Of Tsukuba ; (present Office)national Grassland Researc
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Ohmomo Sadahiro
草地試験場
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NISHIZAKI YOUSUKE
Laboratory of Applied Microbial Genetics, Faculty of Agriculture, Graduate Schools, Kyushu Universit
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UMEDA AKIKO
Department of Basic Laboratory Sciences, Faculty of Health Sciences, Yamaguchi University School of
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Umeda A
Department Of Basic Laboratory Sciences Faculty Of Health Sciences Yamaguchi University School Of Me
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Umeda Akiko
Department Of Bacteriology Faculty Of Medicine Kyushu University
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Nishizaki Yousuke
Laboratory Of Applied Microbial Genetics Faculty Of Agriculture Graduate Schools Kyushu University
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Umeda A
Department Of Health Science School Of Medicine Yamaguchi University
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