Roles of a 20kDa Protein Associated with a Carbocycle-Forming Enzyme Involved in Aminoglycoside Biosynthesis in Primary and Secondary Metabolism

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概要

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• 2-Deoxy-scyllo-inosose (DOI) synthase participates in the biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotics. The enzyme is expected to be of industrial use, because it converts a sustainable resource (glucose 6-phosphate) into carbocycle (DOI), which easily aromatizes to yield catechol. In the present study, we clarified the physiological role of a non-catalytic 20 kDa protein, BtrC2, associated with DOI synthase from the butirosin-producer Bacillus circulans. Based on the results of complementation analysis using btrC2 disruptant and western analysis against catalytic 40 kDa protein (BtrC) and BtrC2, it is suggested that BtrC2 has two functions. It is involved in the vitamin B6 biosynthesis in primary metabolism, like homologous protein (Pdx2) in Bacillus subtilis. Additionally, it takes part in butirosin biosynthesis as stabilizer of DOI synthase by forming a heterodimer with BtrC, and qualifies B. circulans for stable and constant production of butirosin for long periods.

• 2010-06-23

著者

• Eguchi Tadashi

  Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.

• Eguchi T

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Eguchi Tadashi

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Eguchi T

  Tokyo Inst. Technol. Tokyo

• NANGO Eriko

  Department of Chemistry, Tokyo Institute of Technology

• Sawada Hiroshi

  Department of General Studies, College of Humanities and Sciences, Nihon University

• HIRAKAWA Haruka

  Department of General Studies, College of Humanities and Sciences, Nihon University

• IINO Teruhiko

  Department of General Studies, College of Humanities and Sciences, Nihon University

• Eguchi T

  Kyushu Univ. Fukuoka Jpn

• Iino Teruhiko

  Dep. Of General Studies Coll. Of Humanities And Sciences Nihon Univ.

• Iino Teruhiko

  Department Of General Education College Of Humanities And Science Nihon University

• Sawada Hiroshi

  Department Of General Studies Nihon University

• Eguchi T

  Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.

• Tamegai Hideyuki

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Aoki Rie

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Sawada Hiroshi

  Department Of General Studies College Of Humanities And Sciences Nihon University

• Nango Eriko

  Department Of Chemistry Graduate School Of Science And Engineering Tokyo Institute Of Technology

• Hirakawa Haruka

  Department Of General Studies College Of Humanities And Sciences Nihon University

• Eguchi Tadashi

  Department Of Chemistry & Materials Science Tokyo Institute Of Technology

• Iino Teruhiko

  Department of Chemistry, Nihon University

• Sawada Hiroshi

  Department of Agricultural Chemistry, Shimane University

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