Identification of Aminotransferase Genes for Biosynthesis of Aminoglycoside Antibiotics from Soil DNA

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概要

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• Aminoglycoside has been known as a clinically important antibiotic for a long time, but genetic information for the biosynthesis of aminoglycoside is still insufficient. In this study, we tried to clone aminoglycoside-biosynthetic genes from soil DNA for accumulation of genetic information. We chose the genes encoding L-glutamine:(2-deoxy-)scyllo-inosose aminotransferase as the target, because it is specific for all types of aminoglycoside biosynthesis. By degenerate PCR, we obtained 33 individual clones that were homologous with aminotransferase genes in aminoglycoside biosynthesis. Phylogenetic analysis and alignment of these genes showed that horizontal gene transfer has occurred in the soil. Among these, several quite interesting genes were obtained. Some genes probably originated from non-actinomycetes, and some were far from the known homologs. These genes can be useful markers for the isolation of entire gene clusters and originating organisms.

• 社団法人 日本農芸化学会の論文
• 2005-07-23

著者

• Tamegai Hideyuki

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• Tamegai Hideyuki

  Dep. Of Chemistry Coll. Of Humanities And Sciences Nihon Univ.

• NAGAYA Atsushi

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• TAKEYAMA Satoko

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• Nagaya Atsushi

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Tamegai Hideyuki

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Takeyama Satoko

  Department Of Chemistry College Of Humanities And Sciences Nihon University

関連論文

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• Roles of a 20 kDa Protein Associated with a Carbocycle-Forming Enzyme Involved in Aminoglycoside Biosynthesis in Primary and Secondary Metabolism
• Physiological Roles of Two Dissimilatory Nitrate Reductases in the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1
• Biosynthesis of 2-Deoxystreptamine-containing Antibiotics in Streptoalloteichus hindustanus JCM 3268 : Characterization of 2-Deoxy-scyllo-inosose Synthase
• Extended Sequence and Functional Analysis of the Butirosin Biosynthetic Gene Cluster in Bacillus circulans SANK 72073
• First Identification of Streptomyces Genes Involved in the Biosynthesis of 2-Deoxystreptamine-containing Aminoglycoside Antibiotics : Genetic and Evolutionary Analysis of L-Glutamine : 2-deoxy-scyllo-inosose Aminotransferase Genes
• Identification of L-Glutamine : 2-Deoxy-scyllo-inosose Aminotransferase Required for the Biosynthesis of Butirosin in Bacillus circulans
• Novel Substrate Specificity of Designer 3-Isopropylmalate Dehydrogenase Derived from Thermus thermophilus HB8(Biochemistry & Molecular Biology)
• Butirosin-biosynthetic Gene Cluster from Bacillus circulans
• Identification of the Functional Periplasmic Nitrate Reductase (nap) Gene Cluster from the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1
• Significance of the 20-kDa Subunit of Heterodimeric 2-Deoxy-scyllo-inosose Synthase for the Biosynthesis of Butirosin Antibiotics in Bacillus circulans(Microbiology & Fermentation Technology)
• Molecular Cloning of the Gene for the Key Carbocycle-forming Enzyme in the Biosynthesis of 2-Deoxystreptamine-containing Aminocyclitol Antibiotics and Its Comparison with Dehydroquinate Synthase
• Purification and Characterization of 2-Deoxy-scyllo-inosose Synthase Derived from Bacillus circulans. A Crucial Carbocyclization Enzyme in the Biosynthesis of 2-Deoxystreptamine-containing Aminoglycoside Antibiotics
• Identification of Aminotransferase Genes for Biosynthesis of Aminoglycoside Antibiotics from Soil DNA
• Piezo-Adapted 3-Isopropylmalate Dehydrogenase of the Obligate Piezophile Shewanella benthica DB21MT-2 Isolated from the 11,000-m Depth of the Mariana Trench
• Open Reading Frame 3 of the Barotolerant Bacterium Strain DSS12 Is Complementary with cydD in Eschericahia coli: cydD Functions Are Required for Cell Stability at High Pressure
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• Thermal Stability of Cytochrome c5 of Pressure-Sensitive Shewanella livingstonensis
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• Thermal Stability of Cytochrome c_5 of Pressure-Sensitive Shewanella livingstonensis
• The Respiratory System of the Piezophile Photobacterium profundum SS9 Grown under Various Pressures
• Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis
• Regulation of Cytochrome c- and Quinol Oxidases, and Piezotolerance of Their Activities in the Deep-Sea Piezophile Shewanella violacea DSS12 in Response to Growth Conditions
• Regulation of Cytochrome c- and Quinol Oxidases, and Piezotolerance of Their Activities in the Deep-Sea Piezotolerance of Their ctivies in the Deep-Sea Piezophile Shewanella violacea DSS12 in Response to Growth Conditions
• Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis

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