Identification and Diversity of Putative Aminoglycoside-Biosynthetic Aminotransferase Genes from Deep-Sea Environmental DNA
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概要
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We obtained DNA fragments encoding putative aminotransferases possibly involved in the biosynthesis of aminoglycoside antibiotics from deep-sea sediments of the northwest Pacific Ocean by nested PCR, and 34 individual genes (total 89 clones) were identified. About half of the deep-sea sequences showed similarity with genes of known aminoglycoside-producers, but others were deep-sea specific genes. Furthermore, we found that temperature-gradient gel electrophoresis (TGGE) can be an effective tool in the analysis of these DNA fragments.
- 2008-05-23
著者
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AOKI Rie
Department of Preventive Medicine, Nagoya University School of Medicine
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Tamegai Hideyuki
Department of Chemistry, College of Humanities and Sciences, Nihon University
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Aoki Rie
Dep. Of Chemistry Coll. Of Humanities And Sciences Nihon Univ.
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Kato Chiaki
Extremobiosphere Research Center, JAMSTEC
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NAGAYA Atsushi
Department of Chemistry, College of Humanities and Sciences, Nihon University
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Nagaya Atsushi
Department Of Chemistry College Of Humanities And Sciences Nihon University
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ARAKAWA Shizuka
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
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Kato Chiaki
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Tamegai Hideyuki
Department Of Chemistry College Of Humanities And Sciences Nihon University
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Aoki Rie
Department Of Chemistry College Of Humanities And Sciences Nihon University
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Arakawa Shizuka
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Arakawa Shizuka
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology
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