Piezo-Adapted 3-Isopropylmalate Dehydrogenase of the Obligate Piezophile Shewanella benthica DB21MT-2 Isolated from the 11,000-m Depth of the Mariana Trench
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概要
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3-Isopropylmalate dehydrogenase (IPMDH)-encoding leuB genes were obtained from the obligate piezophile Shewanella benthica DB21MT-2 and non-piezophile Shewanella oneidensis MR-1. The genes were expressed in Escherichia coli and the proteins were purified using His-tag. The estimated kinetic parameters of these enzymes indicated that IPMDH of S. benthica DB21MT-2 is more tolerant of high pressure than that of S. oneidensis MR-1. Thus such an adaptation is one of the mechanisms bacteria utilize for survival at high pressures.
- 社団法人 日本農芸化学会の論文
- 2009-11-23
著者
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Tamegai Hideyuki
Department of Chemistry, College of Humanities and Sciences, Nihon University
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SATO Takako
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
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Kasahara Ryota
Department of Chemistry, College of Humanities and Sciences, Nihon University
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Sato Takako
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology
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Kasahara Ryota
Department Of Chemistry College Of Humanities And Sciences Nihon University
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Kato Chiaki
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Tamegai Hideyuki
Department Of Chemistry College Of Humanities And Sciences Nihon University
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Sato Takako
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Kato Chiaki
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology
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