Roles of a 20 kDa Protein Associated with a Carbocycle-Forming Enzyme Involved in Aminoglycoside Biosynthesis in Primary and Secondary Metabolism
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概要
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2-Deoxy-scyllo-inosose (DOI) synthase participates in the biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotics. The enzyme is expected to be of industrial use, because it converts a sustainable resource (glucose 6-phosphate) into carbocycle (DOI), which easily aromatizes to yield catechol. In the present study, we clarified the physiological role of a non-catalytic 20 kDa protein, BtrC2, associated with DOI synthase from the butirosin-producer Bacillus circulans. Based on the results of complementation analysis using btrC2 disruptant and western analysis against catalytic 40 kDa protein (BtrC) and BtrC2, it is suggested that BtrC2 has two functions. It is involved in the vitamin B6 biosynthesis in primary metabolism, like homologous protein (Pdx2) in Bacillus subtilis. Additionally, it takes part in butirosin biosynthesis as stabilizer of DOI synthase by forming a heterodimer with BtrC, and qualifies B. circulans for stable and constant production of butirosin for long periods.
著者
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AOKI Rie
Department of Preventive Medicine, Nagoya University School of Medicine
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Tamegai Hideyuki
Department of Chemistry, College of Humanities and Sciences, Nihon University
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EGUCHI Tadashi
Department of Chemistry and Materials Science, Tokyo Institute of Technology
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NANGO Eriko
Department of Chemistry, Tokyo Institute of Technology
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Sawada Hiroshi
Department of General Studies, College of Humanities and Sciences, Nihon University
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HIRAKAWA Haruka
Department of General Studies, College of Humanities and Sciences, Nihon University
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IINO Teruhiko
Department of General Studies, College of Humanities and Sciences, Nihon University
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Sawada H
Ntt Communication Sci. Lab. Kyoto Jpn
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Sawada Hiroshi
Department Of General Studies Nihon University
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