Comparative Analysis of Highly Homologous Shewanella Cytochromes c_5 for Stability and Function
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概要
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Homologous cytochromes c5 from a mesophile, Shewanella amazonensis (SA cytc5), and a psychrophile, Shewanella violacea (SV cytc5), were compared to elucidate the molecular mechanisms underlying protein stability and function. Cyclic voltammetry revealed that the two proteins had the same redox potential value. Differential scanning calorimetry showed that SV cytc5 was more stable than SA cytc5 in an enthalpic manner. These results and the structure model of Shewanella oneidensis cytochrome c5 indicated that hydrophobic heme environments in the two proteins are the same to maintain the same redox potential value, and that the intra-molecular interactions in SV cytc5, perhaps involved in Lys-50 and Tyr-73, account for its higher stability. Electron transfer from SV cytc5 to membrane proteins of S. violacea and S. amazonensis was faster than that from SA cytc5, suggesting that solvent-exposed Lys-4 in SV cytc5 is responsible for the faster association and dissociation between SV cytc5 and its redox partner.
- 2010-05-23
著者
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Sambongi Yoshihiro
Graduate School Of Biosphere Sci. Hiroshima Univ. Crest Of Japan Sci. And Technol. Corp.
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Tamegai Hideyuki
Department of Chemistry, College of Humanities and Sciences, Nihon University
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Uchiyama Susumu
Graduate School Of Engineering Osaka University
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Wakai Satoshi
Graduate School Of Biosphere Science Hiroshima University
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Wakai Satoshi
Graduate School Of Biosphere Science Hiroshima University Crest Of Japan Science And Technology Corp
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Tamegai Hideyuki
Department Of Chemistry College Of Humanities And Sciences Nihon University
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Takenaka Satoshi
Graduate School Of Biosphere Science Hiroshima University Crest Of Japan Science And Technology Corp
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Sambongi Yoshihiro
Dep. Of Biofunctional Sci. And Technol. Graduate School Of Biosphere Sci. Hiroshima Univ.
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Sambongi Yoshihiro
Graduate School Of Biosphere Science Hiroshima University Crest Of Japan Science And Technology Corp
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Sambongi Yoshihiro
Department Of Biofunctional Science And Technology Graduate School Of Biosphere Science Hiroshima Un
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