High Thermal Stability and Unique Trimer Formation of Cytochrome c′ from Thermophilic Hydrogenophilus thermoluteolus
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概要
- 論文の詳細を見る
Sequence analysis indicated that thermophilic Hydrogenophilus thermoluteolus cytochrome c′ (PHCP) and its mesophilic homolog, Allochromatium vinosum cytochrome c′ (AVCP), closely resemble each other in a phylogenetic tree of the cytochrome c′ family, with 55% sequence identity. The denaturation temperature of PHCP was 87 °C, 35 °C higher than that of AVCP. Furthermore, PHCP exhibited a larger enthalpy change value during its thermal denaturation than AVCP. While AVCP was dimeric, as observed previously, PHCP was trimeric, and this was the first observation as a cytochrome c′. Dissociation of trimeric PHCP and its protein denaturation reversibly occurred at the same time in a two-state transition manner. Therefore, PHCP is enthalpically more stable than AVCP, perhaps due to its unique trimeric form, in addition to the lower number of Gly residues in its putative α-helical regions.
著者
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Yamanaka Masaru
Graduate School Of Biosphere Sci. Hiroshima Univ. Crest Of Japan Sci. And Technol. Corp.
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Sambongi Yoshihiro
Graduate School Of Biosphere Sci. Hiroshima Univ. Crest Of Japan Sci. And Technol. Corp.
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Wakai Satoshi
Graduate School of Biosphere Science, Hiroshima University
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Wakai Satoshi
Graduate School Of Biosphere Science Hiroshima University
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Masanari Misa
Graduate School Of Biosphere Science Hiroshima University
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NISHIHARA Hirofumi
Faculty of Agriculture, Ibaraki University
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FUJII Sotaro
Graduate School of Biosphere Science, Hiroshima University
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INOUE Hiroki
Graduate School of Biosphere Science, Hiroshima University
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