Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis

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概要

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• BtrC2 of the butirosin producer Bacillus circulans is a non-catalytic subunit of 2-deoxy-scyllo-inosose (DOI) synthase that is involved in butirosin biosynthesis, and also a homolog of glutamine amidotransferase subunit (PdxT) of pyridoxal 5'-phosphate (PLP) synthase of Bacillus subtilis. BtrC2 has been found to have functions in B. circulans both in primary and secondary metabolism. In this study, we investigated the properties of PdxT of B. subtilis in order to determine whether the property of enzyme stabilization is universal among PdxT homologs. Complementation with PdxT in the btrC2 disruptant of B. circulans restored the growth and short-term production of antibiotics, but long-term production of antibiotics cannot be restored. Additionally, PdxT did not bind physically with or stabilize BtrC. Our results indicate that the function of BtrC2 in secondary metabolism is specific properties, not universal among PdxT homologs.

著者

• Tamegai Hideyuki

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Sawada Hiroshi

  Department Of General Studies College Of Humanities And Sciences Nihon University

• Eguchi Tadashi

  Department Of Chemistry & Materials Science Tokyo Institute Of Technology

• Haga Minami

  Department Of Correlative Study In Physics And Chemistry Graduate School Of Integrated Basic Science

• Oikawa Yuji

  Department Of Cardiology Cardiovascular Institute

• ITAGAKI Shiori

  Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• OIKAWA Yuji

  Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• SAKODA Ayaka

  Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• OHKE Yoshie

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• SAKODA Ayaka

  Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• HAGA Minami

  Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• SAWADA Hiroshi

  Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University

• Sawada Hiroshi

  Department of Agricultural Chemistry, Shimane University

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