Synthesis of 2-O-Methyl Ether and 1-Carboxamide Derivatives of (2R,3S)-3-Isopropylmalic Acid and Their Interaction with Thermophilic 3-Isopropylmalate Dehydrogenase
スポンサーリンク
概要
- 論文の詳細を見る
To get a closer insight into the substrate recognition of thermostable 3-isopropylmalate dehydrogenase (IPMDH) derived from Thermus thermophilus HB8, two analogs of the natural substrate, (2R, 3S)-3-isopropylmalic acid (IPM) were synthesized according to the chiral transcription methodology which we have recently developed, and the kinetics of the enzyme reaction were analyzed. 2-O-methyl IPM was found to be inactive as a substrate but to function as an uncompetitive inhibitor, which suggests that, although it is the site of oxidation by NAD ^+, 2-O-methyl IPM was incorporated into one of the active sites of the homodimeric enzyme. The hydroxyl group at C-2 was not essential to the substrate recognition by IPMDH. The 1-carboxamide derivative of IPM was inactive, both as a substrate and as an inhibitor. This clearly implies the prime importance of electrostatic interaction between the C-1 carboxylate of IPM and a cationic function (probably a guanidino group of Arg-104) of the enzyme for the recognition of IPM.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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Oshima Tairo
Department of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Scien
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EGUCHI Tadashi
Department of Chemistry and Materials Science, Tokyo Institute of Technology
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KAKINUMA Katsumi
Department of Chemistry, Tokyo Institute of Technology
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Oshima T
Department Of Life Science Tokyo Institute Of Technology:(present Address)faculty Of Life Science To
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Eguchi Tadashi
Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.
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Eguchi T
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
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Eguchi Tadashi
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
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Eguchi T
Tokyo Inst. Technol. Tokyo
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Kakinuma Katsumi
Tokyo Institute of Technology, Department of Chemistry
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Terasawa Hiroaki
Department of Chemistry, Tokyo Institute of Technology
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Miyazaki Kentaro
Department of Life Science, Tokyo Institute of Technology
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Kakinuma K
Tokyo Inst. Technol. Tokyo
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Kakinuma Katsumi
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
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Oshima Tairo
Department Of Life Science Faculty Of Biocscience And Bioctechnology Tokyo Institute Of Technology
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Miyazaki Kentaro
Department Of Life Science Tokyo Institute Of Technology
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Miyazaki Katsunori
Chemistry Laboratories Nippon Shinyaku Co. Ltd.
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Eguchi T
Dep. Of Chemistry And Materials Sci. Graduate School Of Sci. And Engineering Tokyo Inst. Of Technol.
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Eguchi Tadashi
Department Of Chemistry & Materials Science Tokyo Institute Of Technology
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Terasawa Hiroaki
Department Of Chemistry Tokyo Institute Of Technology
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Kakinuma Katsumi
Department of Chemistry & Materials Science, and Department of Chemistry, Tokyo Institute of Technology
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