Endosymbiotic Methanobrevibacter species Living in Symbiotic Protists of the Termite Reticulitermes speratus Detected by Fluorescent In Situ Hybridization

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概要

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• Two species of cellulolytic protist, Dinenympha parva and Microjoenia, living in the guts of the lower termite Reticulitermes speratus are known to harbor endosymbiotic methanogens detectable with an epifluorescent microscope. DNA isolated from the guts of worker termites in a colony of R. speratus was amplified using archaeaspecific primers and cloned, and partial 16S rRNA gene sequences were obtained. Archaeal PCR clones obtained from the guts of xylophagous insects in this and previous works formed four subgroups within the Methanobrevibacter branch of a phylogenetic tree; the sequences of the clones obtained in this report belonged to subgroups, designated XSAT1A and XSAT1D. Using a probe specific to each of the subgroups, 50 and 10 endosymbiotic Methanobrevibacter cells per protist respectively were detected with a probe specific to the subgroup XSAT1A by fluorescent in situ hybridization analysis in D. parva and Microjoenia. There was no observed hybridization to the endosymbiont with other subtype-specific probes including XSAT1D. Based on these results, endosymbionts in D. parva and Microjoenia sp. are proposed to belong to the Methanobrevibacter subgroup XSAT1A.

• 日本微生物生態学会の論文

著者

• YAMAGISHI Akihiko

  Department of Life Sciences, Tokyo University of Pharmacy and Life Sciences

• Oshima Tairo

  Department of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Scien

• Oshima T

  Tokyo Univ. Pharmacy And Life Sci. Tokyo

• Oshima T

  Tokyo Inst. Technol. Tokyo Jpn

• Oshima Tairo

  Department Of Molecular Biology School Of Life Science Tokyo University Ofpharmacy And Life Science

• Oshima Tairo

  Department Of Life Science Tokyo Institute Of Technology

• Oshima Tairo

  Department Of Life Science Faculty Of Biocscience And Bioctechnology Tokyo Institute Of Technology

• Yamagishi Akihiko

  Ochanomizu University

• Yamagishi Akihiko

  Department Of Molecular Biology School Of Life Science Tokyo University Ofpharmacy And Life Science

• Yamagishi Akihiko

  Department Of Life Science Tokyo Institute Of Technology:(present Address)faculty Of Life Science To

• Shinzato Naoya

  Research Institute Of Biological Resources National Institute Of Advanced Industrial Science And Tec

• HARA KURT

  Department of Molecular Biology, School of Life Science, Tokyo University ofPharmacy and Life Scienc

• Yamagishi Akihiko

  Department Of Chemistry Toho University

• Yamagishi Akihiko

  Department Of Chemistry College Of Arts And Sciences The University Of Tokyo

• Hara Kurt

  Department Of Molecular Biology School Of Life Science Tokyo University Ofpharmacy And Life Science

• YAMAGISHI Akihiko

  Department of Applied Molecular Biology, Tokyo University of Pharmacy and Life Sciences

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