Piezotolerance of the Respiratory Terminal Oxidase Activity of the Piezophilic Shewanella violacea DSS12 as Compared with Non-Piezophilic Shewanella Species

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概要

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• The facultative piezophile Shewanella violacea DSS12 is known to alter its respiratory components under the influence of hydrostatic pressure during growth, suggesting that it has a respiratory system that functions in adaptation to high pressure. We investigated the pressure- and temperature-dependencies of the respiratory terminal oxidase activity of the membrane of S. violacea relative to non-piezophilic Shewanella species. We observed that the activity in the membrane of S. violacea was more resistant to high pressure than those of non-piezophilic Shewanella even though DSS12 was cultured under atmospheric pressure. On the other hand, the temperature dependency of this activity was almost the same for all of the tested strain regardless of optimal growth temperature. Both high pressure and low temperature are expected to lower protein flexibility, causing a decrease in enzyme activity, but the results of this study suggest that the mechanism maintaining enzyme activity under high hydrostatic pressure is different from that at low temperature. Additionally, the responses of the activity to the pressure- and temperature-changes were independent of membrane lipid composition. Therefore, the piezotolerance of the respiratory terminal oxidases of S. violacea is perhaps dependent on the properties of the protein itself and not on the lipid composition of the membrane. Our observations suggest that S. violacea constitutively express piezotolerant respiratory terminal oxidases that serve adaptation to the deep-sea environment.

• 社団法人 日本農芸化学会の論文
• 2011-05-23

著者

• Sambongi Yoshihiro

  Department Of Biotechnology University Of Tokyo:(present Address)isir Osaka University

• Kurihara Tatsuo

  Institute for Chemical Research, Kyoto University

• Tamegai Hideyuki

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• Fujimori Hiroki

  Department Of Applied Chemistry Graduate School Of Engineering Osaka Prefecture University

• Kurihara Tatsuo

  Institute For Chemical Research Kyoto University

• Kato Chiaki

  Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.

• Kawamoto Jun

  Division Of Cardiovascular Surgery National Hospital

• Tamegai Hideyuki

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• Sambongi Yoshihiro

  Dep. Of Biofunctional Sci. And Technol. Graduate School Of Biosphere Sci. Hiroshima Univ.

• Sambongi Yoshihiro

  Graduate School Of Biosphere Science Hiroshima University Crest Of Japan Science And Technology Corp

• Kato Chiaki

  Institute Of Biogeoscience Japan Agency For Marine-earth Science And Technology

• Kawamoto Jun

  Division Of Environmental Chemistry Institute For Chemical Research Kyoto University

• Fujimori Hiroki

  Department Of Chemistry College Of Humanities And Sciences Nihon University

• OTA Yuuya

  Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Scienc

• HAGA Minami

  Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Scienc

• NOGI Yuichi

  Institute of Biogeoscience, Japan Agency for Marine-Earth Science and Technology

• KURIHARA Tatsuo

  Division of Environmental Chemistry, Institute for Chemical Research, Kyoto University

• Kurihara Tatsuo

  Division Of Environmental Chemistry Institute For Chemical Research Kyoto University

• Sambongi Yoshihiro

  Department Of Biofunctional Science And Technology Graduate School Of Biosphere Science Hiroshima Un

• Nogi Yuichi

  Institute Of Biogeoscience Japan Agency For Marine-earth Science And Technology

• Haga Minami

  Department Of Correlative Study In Physics And Chemistry Graduate School Of Integrated Basic Science

• Kato Chiaki

  Institute Of Biogeoscience (biogeos) Japan Agency Of Marine-earth Science And Technology (jamstec)

• Ota Yuuya

  Department Of Correlative Study In Physics And Chemistry Graduate School Of Integrated Basic Science

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