Identification of rpoBC Genes Encoding for β and β' Subunits of RNA Polymerase in a Deep-Sea Piezophilic Bacterium, Shewanella violacea Strain DSS12
スポンサーリンク
概要
- 論文の詳細を見る
RNA polymerase from cells of the deep-sea bacterium Shewanella violacea DSS12 was purified using three chromatographic steps. An in vitro transcription assay indicated that the purified enzyme was σ70 containing RNA polymerase. The enzyme activity was inhibited in the presence of rifampicin when the sensitive domain was targeted. The rpoBC genes encoding for the β and β′ subunits of RNA polymerase were cloned and their nucleotide sequences determined. Expression plasmids, designated pQSVB and pQSVC, to overproduce these proteins were constructed, and the proteins were purified using a Ni2+ affinity column. In vitro reconstitution using all proteins for the holoenzyme (α, β, β′, σ70) was carried out and the activity of the recombinant RNA polymerase was detected.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
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Kawano H
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Nakasone K
School Of Engineering Kinki Univ.
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Kawano Hiroaki
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Kato C
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Kato C
Extremobiosphere Research Center Jamstec
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USAMI Ron
Department of Engineering, Toyo University
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Yoshida Yasuhiko
Department of Applied Chemistry, Faculty of Engineering, Toyo University
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Kato Chiaki
Extremobiosphere Research Center, JAMSTEC
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HORIKOSHI Koki
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
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Abe Fumiyoshi
Japan Agency for Marine-Earth Science and Technology
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NAKASONE Kaoru
Department of Biotechnology and Chemistry, School of Engineering, Kinki University
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ABE Fumiyoshi
Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTE
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Abe Fumiyoshi
Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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Usami R
Department Of Applied Chemistry Faculty Of Engineering Toyo University
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Usami R
Toyo Univ. Saitama Jpn
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Usami Ron
Department Of Applied Chemistry Faculty Of Engineering Toyo University
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Abe Fumiyoshi
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology (jamstec)
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Horikoshi K
Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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Abe Fumiyoshi
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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Nakasone Kaoru
School Of Engineering Kinki Univ.
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Yoshida Yasuhiko
Department Of Applied Chemistry Faculty Of Engineering Toyo University
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Yoshida Y
Department Of Applied Chemistry Faculty Of Engineering Toyo University:bio-nano Electronics Research
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Kato Chiaki
Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.
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Usami Ron
Graduate School Of Interdisciplinary New Science Toyo University
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Kato Chiaki
Marine Biodiversity Research Program Japan Marine Science And Technology Center (jamstec)
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Horikoshi Koki
Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology (jamstec)
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Nakasone Kaoru
Department Of Biotechnology And Chemistry School Of Engineering Kinki University
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HORIKOSHI Koki
Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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