Protein-DNA Interactions under High-Pressure Conditions, Studied by Capillary Narrow-Tube Electrophoresis

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概要

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• The method of electrophoretic mobility shift assay under high-pressure conditions was improved using a high-pressure electrophoresis apparatus with capillary narrow-tube gel. It was found that the protein–DNA complex in the gel was stained as a high-resolution spot with ethidium bromide. Using this method, it was found that the behavior under high-pressure conditions of the protein–DNA complex composed of NtrC protein and its target promoter DNA is important for the pressure-regulated transcription process, and it was confirmed that the complex was dissociated above a pressure of 70 MPa.

• 社団法人 日本農芸化学会の論文
• 2005-07-23

著者

• Kawano H

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• Nakasone K

  School Of Engineering Kinki Univ.

• Kawano Hiroaki

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• Kato C

  Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec

• Kato C

  Extremobiosphere Research Center Jamstec

• USAMI Ron

  Department of Engineering, Toyo University

• Yoshida Yasuhiko

  Department of Applied Chemistry, Faculty of Engineering, Toyo University

• Kato Chiaki

  Extremobiosphere Research Center, JAMSTEC

• HORIKOSHI Koki

  Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology

• Abe Fumiyoshi

  Japan Agency for Marine-Earth Science and Technology

• NAKASONE Kaoru

  Department of Biotechnology and Chemistry, School of Engineering, Kinki University

• ABE Fumiyoshi

  Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTE

• Abe Fumiyoshi

  Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Usami R

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Usami R

  Toyo Univ. Saitama Jpn

• Usami Ron

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Abe Fumiyoshi

  Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology (jamstec)

• Horikoshi K

  Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Abe Fumiyoshi

  Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol. (jamstec)

• Nakasone Kaoru

  School Of Engineering Kinki Univ.

• Yoshida Yasuhiko

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Yoshida Y

  Department Of Applied Chemistry Faculty Of Engineering Toyo University:bio-nano Electronics Research

• Kato Chiaki

  Extremobiosphere Res. Center Japan Agency For Marine-earth Sci. And Technol.

• Usami Ron

  Graduate School Of Interdisciplinary New Science Toyo University

• Kato Chiaki

  Marine Biodiversity Research Program Japan Marine Science And Technology Center (jamstec)

• Horikoshi Koki

  Extremobiosphere Research Center Japan Agency For Marine-earth Science And Technology (jamstec)

• Nakasone Kaoru

  Department Of Biotechnology And Chemistry School Of Engineering Kinki University

• HORIKOSHI Koki

  Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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