Extracellular Production of Alkaline Xylanase of Alkalophilic Bacillus sp. by Escherichia coli Carrying pCX311
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概要
- 論文の詳細を見る
Escherichia coli HB101 carrying a plasmid pCX311 which contained an alkaline xylanase gene of the alkalophilic Bacillus sp. strain C-125 produced large amounts of xylanase. More than 70% of the pCX311-borne xylanase was found in the culture broth. LB-medium was most suitable for the production of extracellular xylanase. The production of the enzyme required the addition of Na^+ and was strongly inhibited by the addition of the glucose. Extracellular xylanase production by E. cole carrying pCX311 reached its maximum in 1/3 the cultivation period required by the alkalophilic Bacillus sp. strain C-125. Furthermore, the activity of pCX311-encoded xylanase detected in the culture broth was higher than that of xylanase produced by the alkalophilic Bacillus sp. strain C-125.
- 社団法人日本生物工学会の論文
- 1986-10-25
著者
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Kudo Toshiaki
Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University
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Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)
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Kawano H
Extremobiosphere Research Center (xbr) Japan Agency For Marine-earth Science And Technology (jamstec
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Takio K
Enviromental Molecular Biology Lab. Riken
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Kudo T
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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Kudo T
Riken Saitama Jpn
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Kudo Toshiaki
International Graduate School Of Arts And Sci. Yokohama City Univ.
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Kudo Toshiaki
Yokohama City Univ. Kanagawa Jpn
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Honda H
Univ. Tsukuba Ibaraki Jpn
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HONDA Hiroshi
The Riken Institute
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KUDO Toshiaki
The Riken Institute
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HORIKOSHI Koki
The Riken Institute
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Horikoshi K
Extremobiosphere Res. Center (xbr) Japan Agency For Marine-earth Sci. And Technol. (jamstec)
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