Genetic Organization and Characterization of the mau Gene Cluster, Which Concerned the Initial Step of Electron Transport Chains Involved in Methylamine Oxidation of the Obligate Methylotroph Methylomonas sp. Strain J
スポンサーリンク
概要
- 論文の詳細を見る
A gene cluster encoding methylamine dehydrogenase (MADH) and its primary electron acceptor, azurin iso-2 (the initial step of electron transport chains involved in methylamine oxidation) of the obligate methylotroph Methylomonas sp. strain J (Methylomonas J) was characterized. PCR products synthesized using primers based on the N- and C-terminal amino acid sequences of MADH light subunit from the facultative methylotroph Methylobacterium extorquens AM1 were used as probes for cloning. Fourteen open reading frames were found in a cloned EcoRI-PstI fragment consisting of a total of 11,549 bp. Eight open reading frames were identified as mauFBEDA GLM based on the high gomology to those from Methylophilus methylotrophs W3A1-NS. The mauA and mauB genes encode L subunit and H subunit of MADH, respectively. The mauO which encodes azurin iso-2,a primary electron acceptor from MADH of Methylomonas J, was located downstream from the mauFBEDAGLM genes and the direction of transcription of this gene was found to be opposite to that of the mau gene cluster. Northern blotting analysis suggested that the expression of the mau gene cluster and the mauO gene is co-regulated. These results suggest that dynamic gene recruitment occurred for the initial step of the electron transport chains involed in methylamine oxidation of methylotrophs.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
-
Kudo Toshiaki
Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University
-
Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)
-
Takio K
Enviromental Molecular Biology Lab. Riken
-
Kudo T
Inst. Physical And Chemical Res.(riken) Saitama Jpn
-
Kudo T
Riken Saitama Jpn
-
Kudo Toshiaki
International Graduate School Of Arts And Sci. Yokohama City Univ.
-
Kudo Toshiaki
Yokohama City Univ. Kanagawa Jpn
-
Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research
-
TAGUCHI KATSUHIKO
Laboratory of Microbiology, The Institute of Physical and Chemical Research(RIKEN)Corporation
-
TOBARI Jiro
Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University
-
Tobari J
Laboratory Of Biochemistry College Of Science Rikkyo (st. Paul's) University
-
Taguchi K
Inst. Physical And Chemical Res.(riken) Saitama Jpn
-
Taguchi Katsuhiko
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)corporation
-
Tobari Jiro
Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University
-
Taguchi Katsuhiko
Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University:Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)
関連論文
- Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins
- Expression of a Novel 90-kDa Protein, Lsd90, Involved in the Metabolism of Very Long-chain Fatty Acid-containing Phospholipids in a Mitosis-defective Fission Yeast Mutant
- PA-42 An alternative pathway of primary metabolism of termite gut protists inferred from meta-EST analysis(Symbiosis/Cell to cell interaction,Poster presentation A)
- Effect of Temporal Sample Preservation on the Molecular Study of a Complex Microbial Community in the Gut of the Termite Microcerotermes sp
- Identification and in situ Detection of Two Lineages of Bacteroidales Ectosymbionts Associated with a Termite Gut Protist, Oxymonas sp.
- Fungal Community Analysis of Fungus Gardens in Termite Nests
- Isolation and Detection of Methanogens from the Gut of Higher Termites
- Gut of Higher Termites as a Niche for Alkaliphiles as Shown by Culture-Based and Culture-Independent Studies
- Nitrogen Fixation Genes Expressed in the Symbiotic Microbial Community in the Gut of the Termite Coptotermes formosanus
- Molecular Phylogeny of Symbiotic Basidiomycetes of Fungus-growing Termites in Thailand and Their Relationship with the Host(Microbiology & Fermentation Technology)
- Diverse Bacteria Related to the Bacteroides Subgroup of the CFB Phylum within the Gut Symbiotic Communities of Various Termites(Microbiology & Fermentation Technology)
- Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)
- Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4
- Cloning, Expression and Nucleotide Sequence of the L-2, 3-Butanediol Dehydrogenase Gene from Brevibacterium saccharolyticum C-1012
- Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
- Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli
- Cloning, Nucleotide Sequence, and Hyperexpression of α-Amylase Gene from an Archaeon, Thermococcus profundus
- Preparation of a Chiral Acetoinic Compound Using Transgenic Escherichia coli Expressing the 2,3-Butanediol Dehydrogenase Gene
- An Alkaliphilic and Xylanolytic Paenibacillus Species Isolated from the Gut of a Soil-Feeding Termite
- A shaE Deletion Mutant Showed Lower Na^+ Sensitivity Compared to Other Deletion Mutants in the Bacillus subtilis Sodium/Hydrogen Antiporter (Sha) System
- Molecular ecology of termite-microorganisms symbiotic systems(Symposium on Various Interactions between Animal and Microorganism)
- Production, and Biological Characterization, of Dideoxygriseorhodin C by a Streptomyces sp. and Its Taxonomy
- Nucleotide Sequence of the Xylanase A Gene of Alkalophilic Bacillus sp. Strain C-125(Microbiology & Fermentation Industry)
- Extracellular Production of Alkaline Xylanase of Alkalophilic Bacillus sp. by Escherichia coli Carrying pCX311
- Extracellular Production of Xylanase L in Escherichia coli using Excretion Vector pEAP2
- Purification and Partial Characterization of Alkaline Xylanase from Escherichia coli Carrying pCX311
- Molecular Cloning and Expression of β-Isopropylmalate Dehydrogenase Gene from Alkalophilic Bacillus in Escherichia coli
- Isolation of the Fragments from Alkalophilic Bacillus sp. DNA Which are Controlled by Temperature, pH or NaCl Concentration in pGR71 CAT Expression System
- Gene Expression and Production of Bacillus No. 170 Penicillinase in Escherichia coli and Bacillus subtilis
- Molecular Cloning of β-isopropylmalate dehydrogenase Gene from Clostridium butyricum M588
- The Effect of pH on Heat-resistance of Spores of Alkalophilic Bacillus No. 2b-2
- Effect of pH and Sodium Ion on Germination of Alkalophilic Bacillus Species
- Plasmids in Alkalophilic Bacillus sp.
- Molecular Cloning, Nucleotide Sequence and Expression in Escherichia coli of Hyperthermophilic Glutamate Dehydrogenase Gene from Thermococcus profundus
- Properties of Glutamate Dehydrogenase and Its Involvement in Alanine Production in a Hyperthermophilic Archaeon, Thermococcus Profundus^1
- Location of F_1 ATPase-like Genes on the Physical Map of the Bacillus subtilis 168 Chromosome
- Crystal Structure of meso-2,3-Butanediol Dehydrogenase in a Complex with NAD^+ and Inhibitor Mercaptoethanol at 1.7 A Resolution for Understanding of Chiral Substrate Recognition Mechanisms
- Carbon mineralization by termites in tropical forests, with emphasis on fungus combs
- Isolation and Characterization of Dibenzofuran-degrading Actinomycetes : Analysis of Multiple Extradiol Dioxygenase Genes in Dibenzofuran-degrading Rhodococcus Species(Biochemistry & Molecular Biology)
- P-405 SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS BY ORGANOPALLADIUM REACTIONS
- Physiological Properties of a Neutralo-sensitive Mutant Derived from Facultative Alkaliphilic Bacillus sp. C-125
- Characterization of a Mutation Responsible for an Alkali-sensitive Mutant, 18224, of Alkaliphilic Bacillus sp. strain C-125
- Characteristics of Alkali-sensitive Mutants of Alkaliphilic Bacillus sp. Strain C-125 That Show Cellular Morphological Abnormalities
- Phylogenetic and Morphological Diversity of Bacteroidales Members Associated with the Gut Wall of Termites
- Two Sets of Biphenyl and PCB Degradation Genes on a Linear Plasmid in Rhodococcus erythropolis TA421
- A Gram-positive Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus eryth-ropolis Strain TA421, Isolated from a Termite Ecosystem
- Purification and Characterization of α-Amylase from Hyperthermophilic Archaeon Thermococcus profundus, Which Hydrolyzes Both α-1, 4 and α-1, 6 Glucosidic Linkages
- Extracellular Production of Human Immunoglobulin ε-Chain/γ1-Chain Chimeric Fc Polypeptide by Escherichia coli
- Extracellular Production of Human Tumor Necrosis Factor-α by Escherichia coli Using a Chemically-synthesized Gene(Biological Chemistry)
- Cloning and Characterization of the Azurin iso-1 Gene, Concerned with the Electron Transport Chain Involved in Methylamine/Methanol Oxidation in the Obligate Methylotroph Methylomonas sp. Strain J
- Genetic Organization and Characterization of the mau Gene Cluster, Which Concerned the Initial Step of Electron Transport Chains Involved in Methylamine Oxidation of the Obligate Methylotroph Methylomonas sp. Strain J
- Physiological Properties of Two Pseudomonas mendocina Strains Which Assimilate Styrene in a Two-Phase (Solvent-Aqueous) System under Static Culture Conditions
- Production of 3-Vinylcatechol and Physiological Properties of Pseudomonas LF-3, Which Can Assimilate Styrene in a Two-phase (Solvent-Aqueous) System
- Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins
- Site-specific incorporation of 4-lodo-L-phenylalanine through opal suppression
- Polychlorinated Biphenyl/Biphenyl Degrading Gene Clusters in Rhodococcus sp. K37, HA99, and TA431 Are Different from Well-Known bph Gene Clusters of Rhodococci