Cloning and Characterization of the Azurin iso-1 Gene, Concerned with the Electron Transport Chain Involved in Methylamine/Methanol Oxidation in the Obligate Methylotroph Methylomonas sp. Strain J

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概要

• 論文の詳細を見る

• Two azurin-type blue copper proteins, which is concerned with the electron transport chain involved in methylamine/methanol oxidation, have been found in the obligate methylotroph Methylomonas sp. strain J. The azurin iso-1 gene was cloned and sequenced to analyze the role in the electron transport chain. PCR products synthesized with primers based on the N-and C-terminal amino acid sequences of azurin iso-1 were used as probes for cloning. One complete open reading frame (the azurin iso-1 gene) and one partial orf (orf1) were found in a cloned Eco105I-HindIII fragment, pMAZ3,with a total of 1066 bp. The gene encoded 148 amino acid residues. The amino acid sequence after Ala-21,deduced from the nucleotide sequence, was identical to that of the azurin iso-1 protein. The gene was in a region separate from the mau gene cluster in the chromosome. Escherichia coli expressed azurin iso-1. The results of northern blotting analysis suggested that expression of the azurin iso-1 gene is regulated by a complex regulatory network controlling oxidation of methylamine or methanol in this strain ; for example, copper ions affected the expression of the azurin iso-1 gene.

• 社団法人日本農芸化学会の論文
• 1998-05-23

著者

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• TAGUCHI KATSUHIKO

  Laboratory of Microbiology, The Institute of Physical and Chemical Research(RIKEN)Corporation

• TOBARI Jiro

  Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University

• Tobari J

  Laboratory Of Biochemistry College Of Science Rikkyo (st. Paul's) University

• Taguchi K

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Taguchi Katsuhiko

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)corporation

• Tobari Jiro

  Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University

• Taguchi Katsuhiko

  Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University:Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN)

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