Isolation and Characterization of Dibenzofuran-degrading Actinomycetes : Analysis of Multiple Extradiol Dioxygenase Genes in Dibenzofuran-degrading Rhodococcus Species(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including Rhodococcus, Microbacterium, and Terrabacter genera. Heretofore, no dibenzofuran-utilizing strain belonging to the genus Microbacterium has been reported. Five extradiol dioxygenase genes (dfdB, and edi1 to 4) of the strain Rhodococcus sp. YK2 were cloned and analyzed. The nucleotide sequence of dfdB gene was almost identical to the bphC1 gene of Terrabacter sp. DPO360, which was involved in dibenzofuran metabolism in this strain. Southern and Northern hybridization analyses using these extradiol dioxygenase genes as probes suggest that the dfdB gene in YK2 was conserved in diverse dibenzofuran-utilizing actinomycetes ; also, the dfdB gene was the only expressed gene among five extradiol dioxygenase genes in the medium containing DF as a sole carbon source. These results suggest that the dfdB gene is important for dibenzofuran metabolism not only in the strain YK2, but also in diverse dibenzofuran-degrading actinomycetes.
- 社団法人日本農芸化学会の論文
- 2002-07-23
著者
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Kudo Toshiaki
Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University
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Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)
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KUDO Toshiaki
Microbiology Laboratory, RIKEN
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Takio Koji
Riken
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Takio Koji
Biomolecular Characterization Division Riken
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Takio K
Enviromental Molecular Biology Lab. Riken
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Kudo T
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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Kudo T
Riken (inst. Physical And Chemical Res.) Wako Jpn
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Kudo T
Bio-recycle Project Japan Science And Technology Agency:environmental Molecular Biology Laboratory R
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Kudo T
Environmental Molecular Biology Laboratory Riken
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Kudo T
Riken Saitama Jpn
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Kudo Toshiaki
International Graduate School Of Arts And Sci. Yokohama City Univ.
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Kudo Toshiaki
Yokohama City Univ. Kanagawa Jpn
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Iida Toshiya
Ecomolecular Biorecycling Science Research Team, RIKEN Advanced Science Institute
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Nakamura Kaoru
Ecomolecular Biorecycling Science Research Team, RIKEN Advanced Science Institute
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IIDA Toshiya
Microbiolgoy Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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MUKOUZAKA Yuki
Microbiolgoy Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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NAKAMURA Kaoru
Microbiolgoy Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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YAMAGUCHI Isamu
Environmental Plant Research Group, RIKEN (The Institute of Physical and Chemical Research)
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Kudo Toshiaki
Microbial Toxicology Laboratory The Institute Of Physical And Chemical Research
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Takio K
Biomolecular Characterization Division Riken
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Iida Toshiya
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Mukouzaka Yuki
Microbiolgoy Laboratory Riken (the Institute Of Physical And Chemical Research):graduate School Of S
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Nakamura K
Ecomolecular Biorecycling Science Research Team Riken Advanced Science Institute
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Iida Toshiya
Environmental Molecular Biology Laboratory Riken Institute
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Yamaguchi Isamu
Environmental Plant Research Group Riken (the Institute Of Physical And Chemical Research)
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