Nucleotide Sequences and Characterization of Genes Encoding Naphthalene Upper Pathway of Pseudomonas aeruginosa PaK1 and Pseudomonas putida OUS82
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概要
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A 12,808-nucleotide containing DNA fragment cloned from naphthalene-utilizing(Nah^+)Pseudomonas aeruginosa PaK1 was analyzed and compared with the genes(pah_<OUS>)of a 14,462-nucleotide DNA fragment from Pseudomonas putida OUS82. The DNA sequence analyses demonstrated that the naphthalene upper-pathway genes and their deduced enzymes were very similar between the two bacteria : nucleotide similarities, 83-93% ; amino acid similarities, 79-95%. These genes were also similar to those of the nah operon of plasmid NAH7 ; in particular, the OUS82 genes were similar to the nah genes, whereas the PaK1 genes were almost identical to the dox genes of Pseudomonas sp.C18. A region homologous with the 84-bp repeated sequence that Eaton(J.Bacteriol., 176, 7757-7762, 1994)has found at a site upstream of he nah operon was found only in a region downstream of the pah_<PaK> gene cluster in PaK1 and on both sides of the pah_<OUS> gene cluster in OUS82. A PaK1 gene, corresponding to an unknown gene(nahQ)in the nah operon, is located between the 1, 2-dihydroxynaphthalene dioxygenase gene and the trans-o-hydroxybenzylindenepyruvate(tHBPA)hydratase-aldolase gene(nahE), and was suggested to be involved in the conversion of naphthalene to salicylate. Just downstream of the pah_<PaK> gene cluster, a portion of a region was identical to one-third of the transposase gene(tnpA)in a phenol-catabolic plasmid pEST1226.
- 社団法人日本生物工学会の論文
- 1999-06-25
著者
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Fukuda Masao
Department Of Bioengineering Faculty Of Engineering Nagaoka University Of Technology
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TAKIZAWA NOBORU
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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IIDA TOSHIYA
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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SAWADA TAKASHI
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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YAMAUCHI KAZUHIRO
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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WANG YUE-WU
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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KIYOHARA HOHZOH
Biotechnology Laboratory, Department of Applied Chemistry, Faculty of Engineering, Okayama Universit
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Yamauchi Kazuhiro
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Iida Toshiya
Ecomolecular Biorecycling Science Research Team, RIKEN Advanced Science Institute
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Wang Yue-wu
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Iida Toshiya
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Takizawa Noboru
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Kiyohara Hohzoh
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Sawada Takashi
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Iida Toshiya
Environmental Molecular Biology Laboratory Riken Institute
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Takizawa Noboru
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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Kiyohara Hohzoh
Biotechnology Laboratory Department Of Applied Chemistry Faculty Of Engineering Okayama University O
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