Enhanced Soybean Infection by the Legume “Super-Virulent” Agrobacterium tumefaciens Strain KAT23
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概要
- 論文の詳細を見る
Agrobacterium tumefaciens KAT23 harbors a nopaline-type Ti plasmid and is “super-virulent” to soybean (Glycine max) and other leguminous plants. The right and left border sequences of the essential cis-element for T-DNA transfer were removed in order to utilize the high infectivity of this strain in an Agrobacterium-mediated soybean transformation system. The resulting strain, named Soy2, showed no oncogenic activity. After inoculation with disarmed Soy2 harboring binary vector pIG121-Hm and pCAMBIA-WR, soybean epicotyls exhibited high β-glucuronidase activities, with efficiencies higher than EHA105, an A. tumefaciens strain widely used in making transgenic plants.
- 社団法人 日本農芸化学会の論文
著者
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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TANAKA Hiroshi
National Cardiovascular Center
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Koga-ban Yasunori
National Inst. Of Agrobiological Sciences
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KAKU Hisatoshi
National Institute of Agrobiological Resources (NIAR)
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Yukawa Kiyoshi
Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo
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