Modification of Rice Plant Height by Suppressing the Height-Controlling Gene, D18, in Rice

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概要

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• Gibberellin (GA) 3β-hydroxylase catalyzes the final step of active GA synthesis in plants. In rice, the enzyme is encoded by at least two genes (OsGA3ox1 and OsGA3ox2) with differential patterns of expression. Previously, we reported that the OsGA3ox2 gene is identical with the D18 gene. The loss-of-function mutants of D18 designated as d18-AD (Akibare waisei), d18-1d18^h (housetsu waisei), and d18-dy (Waito-C) show severe dwarfism caused by the decrease of the level of active GA, GA_1. The gene encodes GA 3β-hydroxylase that catalyzes the reaction from GA_<20> to GA_1 and its expression is also regulated by active GA through a feedback mechanism, suggesting that the step catalyzed by the enzyme may be a key step for regulating the level of active GA. To determine the possibility of reducing the height of rice through regulation of the level of active GA, we produced transgenic rice plants expressing the antisense of D18 cDNA. Plants expressing the antisense showed a semi-dwarf phenotype relative to the control plants carrying the vector only. The antisense plants with the semi-dwarf phenotype showed a reduced level of D18 mRNA. In contrast to the reduced level of D18 mRNA, the plants induced a higher expression level of OsGA20ox1, which encodes GA 20-oxidase catalyzing the step from GA_<53> to GA_<20> in the GA biosynthetic pathway. These results demonstrate that the height of rice can be controlled by manipulation of the GA 3β-hydroxylase expression through the decrease in the active GA level.

• 日本育種学会の論文

著者

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya Univ.

• TANAKA Hiroshi

  National Cardiovascular Center

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya University

• ASHIKARI Motoyuki

  Bioscience and Biotechnology Center, Nagoya University

• Itoh Hironori

  BioScience Center, Nagoya University

• Ueguchi-Tanaka Miyako

  BioScience Center, Nagoya University

• Sakamoto Tomoaki

  National Institute of Agrobiological Sciences

• Kayano Toshiaki

  National Institute of Agrobiological Sciences

• MIYAO-TOKUTOMI Mitsue

  National Institute of Agrobiological Resources

• Matsuoka M

  Bioscience And Biotechnology Center Nagoya University

• Ashikari M

  Bioscience And Biotechnology Center Nagoya University

• Ashikari Motoyuki

  Bio-oriented Technology Research Advancement Institution:(present Address)bioscience Center Nagoya U

• Kamiya Takashi

  Chichibu Onoda Cement Corporation Tsukuba Biotechnology R&d Center

• Kayano Toshiaki

  National Institute Of Agrobiological Resources

• Matsuoka Makoto

  Okinawa Subtropical Station Japan International Research Center For Agricultural Sciences

• Sakamoto Tomoaki

  National Institute Of Agrobiological Sciences:(present Address)field Production Science Center Gradu

• KAMIYA Takashi

  Faculty of Engineering, Toyama University

• Saito T

  National Institute Of Fruit Tree Science

• Matuoka Makoto

  Biosci. Cen. Nagoya University

• Matsuoka M

  Institute Of Agriculture And Forestry University Of Tsukuba

• Matsuoka Makoto

  Institute Of Agriculture And Forestry University Of Tsukuba

• Matsuoka Makoto

  Biosci. Center Nagoya Univ. Nagoya

• Ashikari Motoyuki

  Bioscience And Biotechnology Center Nagoya Univ.

• Kayano T

  National Institute Of Agrobiological Sciences

• Ueguchi Miyako

  Bioscience Ctr. Nagoya Univ.

• Miyao‐tokutomi Mitsue

  Niar Tsukuba.

• Sakamoto Tomoaki

  Inst. Agr. & Forest. Univ. Tsukuba

• Tanaka Hiroshi

  National Institute Of Agrobiological Sciences

• Tanaka H

  National Institute Of Agrobiological Sciences

• Ueguchi(Tanaka) Miyako

  BioScience Center, Nagoya University

• ITOH Hironori

  Bioscience and Biotechnology Center, Nagoya University

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