Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene

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概要

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• Sucrose-phosphate synthase (SPS) activity in source leaves of potato plants (Solanum tuberosum cv. May Queen) transformed with maize SPS gene under the control of a modified cauliflower mosaic virus (CaMV) 35S promoter was two to four times higher than the endogenous activity found in the control. A negative correlation (r=0.82) between SPS activity and the ratio of starch to sucrose was observed. There were no significant changes in starch synthesis enzyme (ADGPase and QE) activities in the leaves. Photosynthesis did not differ significantly between control and transgenic lines. Aboveground (leaves and stem) and belowground (tuber) dry matter in three transgenic lines harvested during tuber development increased up to 15% and 20%, respectively, indicating that increased capacity for sucrose synthesis in potato leaves may lead to favorable growth. Moreover, the activity of this enzyme in source leaves of two transgenic potatoes increased when reaction temperature was elevated from 15℃ to 35℃, a response similar to that of SPS in maize. Since temperature is a limiting factor for growth of potato, the transgenic plants will be useful for future studies under higher temperature growth conditions.

• 日本作物学会の論文

著者

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya University

• MATSUOKA Makoto

  Nagoya Univ. BioSci. Cen.

• MIYAO-TOKUTOMI Mitsue

  National Institute of Agrobiological Resources

• Matsuoka M

  Bioscience And Biotechnology Center Nagoya University

• Ohsugi R

  National Institute Of Agrobiological Sciences:(present Office) Graduate School Of Agricultural And L

• Ohsugi Ryu

  National Institute Of Agrobiological Resources

• Tobias Dennis

  National Institute Of Agrobiological Resources (niar)

• Itoh Takafumi

  Div. of Appl. Life Sci., Grad. Sch. of Agriculture, Kyoto Univ.

• Ohsugi Ryu

  Niar

• Itoh Takafumi

  Div. Of Appl. Life Sci. Grad. Sch. Of Agriculture Kyoto Univ.

• Matsuoka Makoto

  Nagoya City Rehabilitation Center

• Hirose Tatsuroh

  Hokuriku National Agricultural Experiment Station

• Ishimaru Ken

  Hokuriku National Agricultural Experiment Station

• Ishige Teruo

  Japan International Research Center for Agricultural Sciences (JIRCAS)

• Ohkawa Yasunobu

  Japan International Research Center for Agricultural Sciences (JIRCAS)

• Kano-Murakami Yuriko

  National Institute of Fruit Tree Science

• Ohsugi Ryu

  Nagoya University

• OHKAWA Yasunobu

  National Institute of Agrobiological Resources

• Ishimaru Ken

  National Institute Of Agrobiological Sciences

• Ishimaru Ken

  N.i.a.r.

• Ishige T

  Japan International Research Center For Agricultural Sciences (jircas)

• ISHIMARU Ken

  Nat. Inst. Agrobiol. Resources

• Ohkawa Y

  National Institute Of Agrobiological Resources

• Hirose T

  Hokuriku National Agricultural Experiment Station

• Kano‐murakami Y

  National Institute Of Fruit Tree Science

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