The Promoter for the Maize C_4 Pyruvate, orthophosphate Dikinase Gene Directs Cell- and Tissue-Specific Transcription in Transgenic Maize Plants

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概要

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• The pyruvate, orthophosphate dikinase (PPDK) gene coding the chloroplast enzyme involved in C_4 photosynthesis has a dual promoter system. The first promoter is responsible for the transcription of a larger transcript and its product is targeted to the chloroplast (hence, it is designated as C4Pdk promoter) while the second promoter is responsible for the transcription of a smaller transcript and its product remains in the cytosol. In this study, chimeric maize C4Pdk promoter (0.9 or 1.5 kb)-β-glucuronidase or luciferase fusion genes were introduced into maize plants by Agrobacterium-mediated transformation. The Cell- and tissue-specificities of the maize C4Pdk promoter in the transgenic maize plants were examined by histochemical and enzymic activity analyses of the reporters in different photosynthetic cells and tissues. The results showed that the reporter proteins are almost exclusively localized in leaf mesophyll cells. Among the tissues tested, leaf blade had the highest reporter activities with sheath exhibiting about 10% of the activities in blade. Husk, stem, tassel and root had no or very little reporter activities. Taken together, these results suggest that the maize C4Pdk promoter is specifically transcribed in the mesophyll cells of leaf blade and to a much less extent in the mesophyll cells of sheath, but not in leaf bundle sheath cells or other tissues. Furthermore, the 0.9 kb maize C4Pdk promoter sequences appear to contain the necessary cis-acting elements for its cell- and organ-specific expression.

• 日本植物生理学会の論文

著者

• Matsuoka Makoto

  Bioscience Center Nagoya University

• SUGIYAMA Tatsuo

  Department of Energy Science and Nuclear Engineering, Tokyo City University

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya University

• ISHIDA Yuji

  Japan Tobacco Inc.

• OHTA Shozo

  Japan Tobacco Inc.

• KOMARI Toshihiko

  Japan Tobacco Inc.

• TANIGUCHI Mitsutaka

  Department of Biological Mechanisms and Function, Nagoya University

• Izawa Katsura

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago

• Ku Maurice

  Department of Botany, Washington State University

• Lin Jenq-Hong

  Department of Botany, National Chung-Hsing University

• Saito Hideaki

  Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.

• Ishida Yuji

  Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.

• Ohta Shozo

  Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.

• Komari Toshihiko

  Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.

• Ohta S

  Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.

• Ohta Shozo

  Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.

• Ishida Y

  Plant Innovation Center Japan Tobacco Inc.

• Matsuoka Makoto

  Biosci.center Nagoya Univ.

• Sugiyama T

  Graduate School Of Bioagricultural Sciences Nagoya University

• Sugiyama Tatsuo

  Dept.appl.biol.sci. Sch.agr.sci. Nagoya Univ.

• Ku M

  Washington State Univ. Wa Usa

• Matsuoka Makoto

  Nagoya City Rehabilitation Center

• Komari Toshihiko

  Plant Innovation Center Japan Tobacco Inc.

• 杉山 長美

  Tohoku Univ. Sendai Jpn

• Sugiyama Tatsuo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Taniguchi Mitsutaka

  Fac. Agri. Nagoya Univ.

• Ku Maurice

  Department Of Botany Washington State University

• Izawa Kyoko

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Lin Jenq-hong

  Department Of Botany National Chung-hsing University

• Saito Hiroyoshi

  Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.

• Matuoka Makoto

  Biosci. Cen. Nagoya University

• Matsuoka Makoto

  Biosci. Center Nagoya Univ. Nagoya

• Komori Toshiyuki

  Genetics Research Laboratory Japan Tobacco Inc.

• Sugiyama Tatsuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University

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