Effectiveness of Sonication on Diminishing Bacterial Overgrowth in Agrobacterium-mediated Transformation

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概要

• 論文の詳細を見る

• This study was carried out to trace the biological effects of exposing Agrobacterium tumefaciens and sugarcane cell clumps of suspension culture to ultrasonic waves. A diluted suspension of Agrobacterium tumefaciens, strains EHA101 and LBA4404, was treated with 44 kHz sonication which remarkably reduced the frequency of colony formation in the inoculated medium of both strains. The cell clumps of the suspension culture were co-cultured with Agrobacterium, and then sonicated. Following 3 days repeating co-culture in solid medium, the cell clumps were transferred to the MS-1e medium. Without sonication treatment, 31% of cell clumps revealed bacterial overgrowth, whereas no overgrowth was found in the sonication treatment after 14 days of culture. However, no significant differences in the rates of growth and shoot regeneration between the sonication-treated cell clumps and the untreated cell clumps.

• 日本植物細胞分子生物学会の論文
• 2004-03-01

著者

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya Univ.

• Matsuoka Makoto

  Bioscience And Biotechnology Center Nagoya University

• MIYAO-TOKUTOMI Mitsue

  National Institute of Agrobiological Resources

• Matsuoka M

  Bioscience And Biotechnology Center Nagoya University

• Tamura Yasuaki

  Okinawa Subtropical Station Japan International Research Center For Agricultural Sciences

• Matsuoka Makoto

  Okinawa Subtropical Station Japan International Research Center For Agricultural Sciences

• Arifin Noor

  Plant Breeding Laboratory Okinawa Subtropical Station Japan International Research Center For Agricu

• Tabiki Tadashi

  National Agricultural Research Center For The Hokkaido Region (memuro)

• Tabiki Tadashi

  Wheat Breeding Lab. Dep. Of Upland Agriculture National Agricultural Res. Center For Hokkaido Region

• Matsuoka M

  Institute Of Agriculture And Forestry University Of Tsukuba

• TERAUCHI Takayoshi

  Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agri

• TAMURA Yasuaki

  Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agri

• TANIO Masahiko

  Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agri

• MATSUOKA Makoto

  Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agri

• Matsuoka Makoto

  Biosci. Center Nagoya Univ. Nagoya

• Tanio Masahiko

  National Agricultural Research Center For The Hokkaido Region (memuro)

• Terauchi Takayoshi

  Plant Breeding Laboratory Okinawa Subtropical Station Japan International Research Center For Agricu

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