Immuno-Gold Localization of Nitrogenase in Root Nodules of Elaeagnus pungens Thunb

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概要

• 論文の詳細を見る

• The molybdenum-iron component of nitrogenase (MO-Fe component) was purified from soy-bean nodule bacteroids and antibody was raised against it in rabbits. Antibody raised against the 53 kDa polypeptide which was the major protein in the Mo-Fe component fraction of soybean nitrogenase was confirmed to be specific to the nitrogenase by immunodiffusion and immuno-titration. The nitrogenase from root nodules of Elaeagnus pungens cross-reacted with the antibody and appeared from the results of the immunodiffusion to be partially identical to soybean nitrogenase. Using the antibody, we examined intracellular localization of nitrogenase in root nodules of Elaeagnus pungens, in which Frankia is present as a symbiont, by immuno-gold labelling. Thin sections of nodules of Elaeagnus pungens were first treated with anti-nitrogenase specific antibody and then with colloidal gold-protein A as a marker. The gold particles were observed to be concentrated in the vesicles of the endophyte Frankia. This provides strong support for the existence of nitrogenase in the vesicles. Furthermore, our results suggested that nitrogenase localizes in the hyphae of the endophyte Frankia in Elaeagnus pungens nodules.

• 日本植物生理学会の論文

著者

• HIYOSHI Toru

  Department of Electronic Science and Engineering, Kyoto University

• SUGIYAMA Tatsuo

  Department of Energy Science and Nuclear Engineering, Tokyo City University

• Sasakawa Hideo

  Department Of Agricultural Chemistry School Of Agriculture Nagoya University

• Hiyoshi Toru

  Department Of Agricultural Chemistry School Of Agriculture Nagoya University:(present)iwata Experime

• Hiyoshi Toru

  Department Of Agricultural Chemistry School Of Agriculture Nagoya University

• Sasakawa Hideo

  Department Of Agricultural Chemistry Nagoya University

• Sugiyama Tatsuo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Sugiyama Tatsuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University

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