Molecular Cloning of the Family of Glutamine Synthetase Genes from Maize: Expression of Genes for Glutamine Synthetase and Ferredoxin-Dependent Glutamate Synthase in Photosynthetic and Non-Photosynthetic Tissues

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Five cDNA clones for glutamine synthetase (GS), pGS107, pGS112, pGS117, pGS122, and pGS202, were isolated from a maize cDNA library constructed with poly(A)^+ RNA from light-induced greening leaves. From a comparison of the primary structures, pGS202 was judged to encode a plastidic isoform, and the others to encode cylosolic isoforms. This result confirms that maize GS is encoded by a small multigene family, as is the case in other plant species. Steady-state levels of proteins and mRNAs for the GS isoforms and ferredoxin-dependent glutamate synthase (Fd-GOGAT) were analysed by Western and Northern blottings in different organs, namely, leaves, mesocotyls and roots, and under various physiological conditions. The levels of both plastidic GS and Fd-GOGAT and those of their respective mRNAs were much higher in photosynthetic organs than in non-photosynthetic organs and increased in a similar manner during illumination of etiolated leaves. Thus, the expression of the plastidic GS and Fd-GOGAT in leaves seems to be coordinately regulated at the transcriptional level. Although the cytosolic isoforms of GS, encoded by the four separate genes could not be quantified separately, the levels of their respective mRNAs were very different from one another. The mRNAs that corresponded to pGS107 and pGS112 were abundant and were distributed almost equally in all organs, while those that corresponded to pGS117 and pGS122 were distributed predominantly in young etiolated leaves and roots, respectively.
日本植物生理学会の論文

Molecular Cloning of the Family of Glutamine Synthetase Genes from Maize: Expression of Genes for Glutamine Synthetase and Ferredoxin-Dependent Glutamate Synthase in Photosynthetic and Non-Photosynthetic Tissues

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