Expression of N-acetylgalactosamine Residues on Ectoderm Cell Surfaces during Neurulation in the Bantam Chick Embryo

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The expression of cell surface N-acetyl-galactosamine (GalNAc), which residues on the ectoderm in the neural plate stage and neural tube stage of bantam embryos, was examined using fluorescein isothiocyanate-labeled lectins BPA, MPA, WFA, HPA, DBA, SJA and LFA before and after neuraminidase treatment. The results showed selective lectin binding on ectoderms of both stages and indicated that the neuroectoderm acquired different carbohydrate compositions from those in the surface-ectoderm during neurulation. The results also may indicate that GalNAc residues are small in quantity at the terminal position on the bantam neurula ectoderm and many of them hold the penultimate position which is masked by sialic acid. The role of masking on the GalNAc residues during neural tube closure is discussed.