Isolation of Dibenzofuran-Degrading Bacterium, Nocardioides sp. DF412, and Characterization of Its Dibenzofuran Degradation Genes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• A dibenzofuran(DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.

• 社団法人日本生物工学会の論文
• 2008-06-25

著者

• Fukuda Masao

  Nagaoka University of Technology

• Sukda Parichat

  Nagaoka University of Technology

• Masai Eiji

  Nagaoka University of Technology

• MASAI Eiji

  Department of Bioengineering, Nagaoka University of Technology

• Masai E

  Department Of Bioengineering Nagaoka University Of Technology

• Masai Eiji

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• Masai Eiji

  Department Of Bioengineering Nagaoka University Of Engineering

• Fukuda Masao

  Department Of Bioengineering Nagaoka University Of Technology

• Fukuda Masao

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• Nishida Tetsuya

  Department Of Internal Medicine Japanese Red Cross Nagoya First Hospital

• Fukuda M

  Nagaoka University Of Technology

• MIYAUCHI Keisuke

  Department of Civil and Environmental Engineering, Tohoku Gakuin University

• Fukuda Masao

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Sukda Parichat

  Department of Bioengineering, Nagaoka University of Technology

• Ito Emi

  Department of Bioengineering, Nagaoka University of Technology

• Matsumoto Yoshikatsu

  Department of Bioengineering, Nagaoka University of Technology

• Nishida Tetsuya

  Department Of Bioengineering Nagaoka University Of Technology

• Miyauchi Keisuke

  Department Of Bioengineering Nagaoka University Of Technology

• Ito Emi

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Miyauchi Keisuke

  Department Of Civil And Environmental Engineering Tohoku Gakuin University

• Ito Emi

  Department Of Bioengineering Nagaoka University Of Technology

• Sukda Parichat

  Department Of Bioengineering Nagaoka University Of Technology

• Nishida Tetsuya

  Department Of Bacteriology Hiroshima University Graduate School Of Biomedical Sciences

• Matsumoto Yoshikatsu

  Department Of Bioengineering Nagaoka University Of Technology

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