Meta-Fission Product Hydrolases from a Strong PCB Degrader Rhodococcus sp. RHA1
スポンサーリンク
概要
- 論文の詳細を見る
Both 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid(HPDA) ana 2-hydroxy-6-oxohepta-2, 4-dienoic acid(HOHD)hydrolases were purified to homogeneity from the cells of PCB-degrader, Rhodococcus sp. RHA1 grown on biphenyl. The NH_2-terminal amino acid sequences of these hydrolases were determined And low homology(35%)was observed between them. Both of them were induced in the RHA1 cells grown on benzene, toluene and ethylbenzene as well as on biphenyl, but not induced in those grown on benzoate, phenol and succinate. The RHA1 HPDA and HOHD hydrolases were indicated to be an octamer and a dimer respectively composed of identical subunits with a molecuhr weight of 33, 000 and 32, 000, respectively. The RHA1 HPDA and HOHD hydrolases were specific for the respective substrates HPDA and HOHD, and the latter also had high activity toward 2-hydroxy muconic semialdehyde(HMSA). Such narrow substrate specificity suggested that HPDA and HOHD hydrolases were responsible for the degradation of biphenyl and toluene, respectively.
- 社団法人日本生物工学会の論文
- 1998-02-25
著者
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Fukuda Masao
Nagaoka University of Technology
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Masai Eiji
Nagaoka University of Technology
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MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
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YAMADA Akihiro
Department of Functional Materials Science, Saitama University
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Masai E
Department Of Bioengineering Nagaoka University Of Technology
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Masai Eiji
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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Fukuda Masao
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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Fukuda M
Nagaoka University Of Technology
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KIYOHARA Hohzoh
Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science
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Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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HATTA TAKASHI
Research Institute of Technology, Okayama University of Science
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SHIMADA TERUHIKO
Research Institute of Technology, Okayama University of Science
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YOSHIHARA TADAHIDE
Research Institute of Technology, Okayama University of Science
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Hatta T
Okayama Univ. Sci. Okayama Jpn
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Hatta Takashi
Research Institute Of Technology Okayama University Of Science
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Shimada Teruhiko
Research Institute Of Technology Okayama University Of Science
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Yoshihara Tadahide
Research Institute Of Technology Okayama University Of Science
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Hirose Toru
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Kiyohara Hohzoh
Department Of Applied Chemistry Faculty Of Engineering Okayama University Of Science
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Yamada A
Department Of Bioengineering Nagaoka University Of Technology
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Yamada Akihiro
Department Of Chemistry Graduate School Of Science
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YAMADA AKIHIRO
Department of Bioengineering, Nagaoka University of Technology
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