Diversity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1
スポンサーリンク
概要
- 論文の詳細を見る
Two 2,3-dihydroxybiphenyl (23DHBP) dioxygenase genes, bphC1 and etbC involved in the degradation of polychlorinated biphenyl(s) (PCBs) have been isolated and characterized from a strong PCB degrader, Rhodococcus sp. RHA1. In this study, four new 23DHBP dioxygenase genes, designated as bphC2, bphC3, bphC4, and bphCS were isolated from RHA1, and their nucleotide sequences were determined. Based on amino acid sequence similarities, all of the newly isolated bphC genes could be categorized into type I along with BphC1 and EtbC [Eltis, L. D. and Bolin, J. T., J. Bacteriol., 178, 5930-5937 (1996)]. Six bphC genes, including bphCl, etbC, and four new genes, were expressed in Eseherichia coli to determine their substrate specificity. The activities of BphC2, BphC3, BphC4, and BphC5 were found to be specific to 23DHBP, while BphC1 and EtbC exhibited activities towards compounds other than 23DHBP, including catechol (CAT) and 3-methylcatechol (3MC). RNA slot blot hybridization analysis indicated that only bphC5 was transcribed among the newly isolated bphC in RHA1 cells grown on biphenyl and ethylbenzene. The nucleotide sequence of the flanking region of each bphC revealed a homolog of the 2-hy-droxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPD) hydrolase gene, bphD, just upstream of bph CS. The bphC5 and putative bphD genes may constitute an operon and play a role in the degradation of biphenyl and PCBs together with bphC1 and eibC. In contrast, the bphC2, bphC3, and bphC4 genes may not be involved in biphenyl and PCB degradation.
- 社団法人日本生物工学会の論文
- 2002-04-25
著者
-
Fukuda Masao
Nagaoka University of Technology
-
Masai Eiji
Nagaoka University of Technology
-
MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
-
Masai E
Department Of Bioengineering Nagaoka University Of Technology
-
Masai Eiji
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
-
Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
-
Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
-
Fukuda Masao
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
-
Fukuda M
Nagaoka University Of Technology
-
Asami H
Nagaoka Univ. Technol. Nagaoka
-
Sakai Miho
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
-
Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
SAKAI Masayuki
Department of Bioengineering, Nagaoka University of Technology
-
ASAMI HIROKI
Department of Bioengineering, Nagaoka University of Technology
-
SUGIYAMA KATSUMI
Department of Bioengineering, Nagaoka University of Technology
-
KIMBARA KAZUHIDE
Japan' and Environmental Biotechnology Laboratory, Rail-way Technical Research Institute
-
Kimbara K
Japan' And Environmental Biotechnology Laboratory Rail-way Technical Research Institute
-
Asami Hiroki
Department Of Bioengineering Nagaoka University Of Technology
-
Kimbara Kazuhide
Japan' And Environmental Biotechnology Laboratory Rail-way Technical Research Institute
関連論文
- EN-O2 Dibenzofuran degradation system in a Nocardioides sp. DF412(Section VI Environmental Biotechnology)
- Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
- Fusible, elastic, and biodegradable polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC)
- Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
- Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
- Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
- Efficiengt Introduction of Vector Plasmids into Acetic Acid Bacteria
- ETHYLENE PRODUCTION BY RECOMBINANT CYANOBACTERIA WHICH POSSESS CARBON DIOXIDE RECYCLING SYSTEM
- Photosynthetic Conversion of Carbon Dioxide to Ethylene by the Recombinant Cyanobacterium, Synechococcus sp. PCC 7942,Which Harbors a Gene for the Ethylene-Forming Enzyme of Pseudomonas syringae
- EN-P40 Insight into the sugar transport and metabolism in Biphenyl/PCB degrader, Rhodococcus jostii RHA1(Section VI Environmental Biotechnology)
- Enzymatic Properties of Terephthalate 1,2-Dioxygenase of Comamonas sp. Strain E6
- Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
- Identification of a Unique 2,3-Dihydroxybiphenyl 1 ,2- Dioxygenase in Rhodococcus strain RHA1 containing a bphC1 disruption
- Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
- Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
- Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
- Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Multiplicity of Aromatic Ring Hydroxylation Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1 Demonstrated by Denaturing Gradient Gel Electrophoresis(Biochemistry & Molecular Biology)
- Complete Degradation of Polychlorinated Biphenyls by a Combination of Ultraviolet and Biological Treatments
- Isolation of Dibenzofuran-Degrading Bacterium, Nocardioides sp. DF412, and Characterization of Its Dibenzofuran Degradation Genes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- 2K10-1 Development of binary vector system for legume transformation based on the soybean-supervirulent Agrobacterium tumefaciens strain KAT23
- Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1
- Diversity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1
- Meta-Fission Product Hydrolases from a Strong PCB Degrader Rhodococcus sp. RHA1
- Nucleotide Sequences and Characterization of Genes Encoding Naphthalene Upper Pathway of Pseudomonas aeruginosa PaK1 and Pseudomonas putida OUS82
- Indigenous Plasmids of Beijerinckia Strains(Microbiology & Fermentation Industry)
- Characterization of polychlorinated biphenyl degradation in a fermentor by Comamonas testosteroni strain TK102
- Specific Heat and Superconductivity of (La_Sr_)_2CuO_4
- Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
- Characterization of a Second 2,3-dihydro-2,3-dihydroxybiphenyl Dehydrogenase Gene of the Polychlorinated Biphenyl Degrader Rhodococcus sp. Strain RHA1
- Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
- A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
- Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
- Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
- Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
- Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
- Increased Bioavailability of Tacrolimus after Rectal Administration in Rats(Biopharmacy)
- Specific Heat and Superconductivity in (La_Ca_x)_2CuO_4
- Organogels of Lignin-derived Stable Metabolic Intermediate, 2-Pyrone-4,6-dicarboxylic Acid (PDC), Bearing Cholesteryl Groups
- Preparation and Characterization of Cr--Zn--N--O Thin Films Deposited by Pulsed Laser Deposition
- Fourier-Transform Infrared Absorption Spectroscopy of Chromium Nitride Thin Film
- Content and Release of Bisphenol A from Polycarbonate Dental Products
- Enhanced Soybean Infection by the Legume “Super-Virulent” Agrobacterium tumefaciens Strain KAT23
- Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Sex Difference in the Cadmium Effect on Pulpal Polyamines in Rat Incisor
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Enhanced Soybean Infection by the Legume "Super-Virulent" Agrobacterium tumefaciens Strain KAT23
- Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Enzymatic Properties of Terephthalate 1, 2-Dioxygenase of Comamonas sp. Strain E6
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- Three-dimensional Structure of Microbial 2-Hydroxy1-6-oxo-6-phenylhexa-2, 4-dienoic Acid (HPDA) Hydrolase (BphD Enzyme) from Rhodococcus sp. Strain RHA1, in the PCB Degradation Pathway
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- POTENTIATION BY PHOSPHATIDYLSERINE OF CALCIUM-DEPENDENT HISTAMINE RELEASE FROM RAT MAST CELLS INDUCED BY CONCANAVALIN A
- SIGNIFICANCE OF ATP-SPLITTING ACTIVITY OF RAT PERITONEAL MAST CELLS IN THE HISTAMINE RELEASE INDUCED BY EXOGENOUS ATP
- Immunoelectron Microscopic Study of Fibronectin on Rat Mast Cells
- CALCIUM-DEPENDENT HISTAMINE RELEASE WITH DEGRANULATION FROM ISOLATED RAT MAST CELLS BY ADENOSINE 5-TRIPHOSPHATE
- HISTAMINE RELEASE FROM ISOLATED RAT MAST CELLS IN METAL ION-FREE MEDIUM
- Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate
- Liquid Crystallinity and Organogelation Behavior of Lignin-Derived Metabolic Intermediate Bearing Cholesterol Groups
- The 24-hp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Construction of Broad Host Range Cloning Vectors for Gram-negative Bacteria
- High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates(METHODS)
- Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation
- Characterization of the Third Glutathione S-Transferase Gene Involved in Enantioselective Cleavage of the β-Aryl Ether by Sphingobium sp. Strain SYK-6
- A COMMON ANTIGEN BETWEEN CANDIDA ALBICANS AND MYCOBACTERIUM TUBERCULOSIS
- High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates
- Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) degradation in Janibacter sp. TYM3221(ENVIRONMENTAL BIOTECHNOLOGY)
- Three-dimensional Structure of 2,3-Dihydroxybiphenyl Dioxygenase (BphC enzyme) from Pseudomonas sp. Strain KKS102 Having Polychlorinated Biphenyl (PCB)-Degrading Activity.
- A simple and rapid assay for collagenase activity using fluorescence-labeled substrate.
- Histamine release induced by proteolytic digests of human serum albumin: Isolation and structure of an active peptide from pepsin treatment.
- Eugenol-mediated superoxide generation and cytotoxicity in guinea pig neutrophils.
- EXPERIMENTAL STUDIES ON THE TREATMENT OF CANDIDIASIS
- Thermoplastic Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of nitrate and nitrite utilization system in Rhodococcus