Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
スポンサーリンク
概要
- 論文の詳細を見る
Pseudomonas paucimobilis UT26 grows on γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. Tn5 mutation was introduced into UT26, and two kinds of mutants defective in γ-HCH degradation were phenotypically isolated; one (UT64) completely lacked the activity to degrade γ-HCH, while the other (UT61) retained a very low level of activity. Tagging and sequencing analysis showed that both mutants had a Tn5 insertion at the same site of the linA (γ-HCH dehydrochlorinase encoding) gene. However, UT61 had an additional rearrangement, which could be the cause of its retaining a low level of activity. An in vitro complementation test with a crude extract from UT64 plus partially purified LinA protein showed that LinA was essential not only for the first-step reaction (γ-HCH to γ-pentachlorocyclohexene; γ-PCCH), but also for the second-step reaction (γ-PCCH to compound B) of γ-HCH degradation in UT26.
- 社団法人日本農芸化学会の論文
- 1993-05-23
著者
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Fukuda Masao
Nagaoka University of Technology
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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SAKAI Akira
Department of Crystalline Materials Science, Graduate School of Engineering, Nagoya University
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Fukuda M
Department Of Bioengineering Nagaoka University Of Technology
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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YANO KEIJI
Department of BioEngineering, Nagaoka University of Technology
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IMAI Ryozo
Department of Low Temperature Sciences, Hokkaido National Agriculture Experimental Station
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Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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NAGATA Yuji
Department of Biotechnology, University of Tokyo
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Yano K
Dept. Bioeng. Nagaoka Univ. Tech.:jrdc
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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IMAI Ryozo
Winter Stress Laboratory, Hokkaido National Agricultural Experiment Station
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Imai Ryozo
Winter Stress Laboratory Hokkaido National Agricultural Experiment Station
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Imai Ryozo
Department Of Low Temperature Sciences Hokkaido National Agriculture Experimental Station
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Nagata Y
Department Of Biotechnology University Of Tokyo
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Nagata Yuji
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
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Sakai Akira
Department Of Agricultural Chemistry The University Of Tokyo
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Nagata Y
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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