The 24-hp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Rhodococcus jostii RHA1 degrades polychiorinated biphenyls (PCBs) by cometabolism with biphenyl. The bphS1T1-coding two-component system, which is composed of a sensor kinase, BphS1, and a response regulator, BphT1, activates the transcription of biphenyl/PCB degradation genes from the five promoters of bphAa, etbAa1, etbAa2, etbAd, and etbD1 in the presence of aromatics, such as biphenyl and ethylbenzene. The transcription start sites of etbAd and etbD1 were detennined and the results indicated that the 18-bp consensus sequence is shared by all five promoters at the equivalent position from their transcriptional start sites. To investigate the involvement of the 18-bp consensus sequence in the regulation of BphS1T1, a hybrid promoter was constructed by connecting the 18-bp consensus sequence of bphAa promoter to a portion of the benzoate dioxygenase gene promoter, which is not under the control of BphS1T1. The ethylbenzene-dependent induction of the hybrid promoter by BphS1T1 was not observed. Recently, a 24-bp consensus sequence that included the 18-bp consensus sequence of the bphAa promoter was identified in the regions conserved among RHA1 and other rhodococcal degraders. When the 24-bp consensus sequence was employed instead, both BphS1T1-dependent basal activation and ethylbenzene-dependent induction of the hybrid promoter were observed. Mutations in the six extra residues outside the 18-bp sequence in the 24-bp consensus sequence, affected not only ethylbenzene-dependent induction but also BphS1T1-dependent basal activation. The outstanding conservation of the 24-bp consensus sequence was confirmed by multiple sequence alignment. These results indicate that the 24-bp consensus sequence is really responsible for the regulation of BphS1T1.
- 公益社団法人日本生物工学会の論文
著者
-
Fukuda Masao
Nagaoka University of Technology
-
Kasai Daisuke
Nagaoka University of Technology
-
MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
-
Masai E
Department Of Bioengineering Nagaoka University Of Technology
-
Masai Eiji
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
-
Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
-
Kasai Daisuke
Nagaoka University Of Technology Department Of Bioengineering
-
Fukuda Masao
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
-
Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Fukuhara Yuki
Department of Bioengineering, Nagaoka University of Technology
-
Miyauchi Keisuke
Department Of Bioengineering Nagaoka University Of Technology
-
Fukuhara Yuki
Department Of Bioengineering Nagaoka University Of Technology
-
Shimodaira Jun
Department Of Bioengineering Nagaoka University Of Technology
-
Miyauchi Keisuke
Department Of Civil And Environmental Engineering Tohoku Gakuin University
-
Kasai Daisuke
Department Of Bioengineering Nagaoka University Of Technology
-
Furusawa Yuki
Department of Bioengineering, Nagaoka University of Technology
-
Miyazawa Yuta
Department of Bioengineering, Nagaoka University of Technology
-
Miyazawa Yuta
Department Of Bioengineering Nagaoka University Of Technology
-
Furusawa Yuki
Department Of Bioengineering Nagaoka University Of Technology
関連論文
- EN-O2 Dibenzofuran degradation system in a Nocardioides sp. DF412(Section VI Environmental Biotechnology)
- Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
- Fusible, elastic, and biodegradable polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC)
- Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
- Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
- Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
- Efficiengt Introduction of Vector Plasmids into Acetic Acid Bacteria
- EN-P40 Insight into the sugar transport and metabolism in Biphenyl/PCB degrader, Rhodococcus jostii RHA1(Section VI Environmental Biotechnology)
- Enzymatic Properties of Terephthalate 1,2-Dioxygenase of Comamonas sp. Strain E6
- Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
- Identification of a Unique 2,3-Dihydroxybiphenyl 1 ,2- Dioxygenase in Rhodococcus strain RHA1 containing a bphC1 disruption
- Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
- Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
- Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
- Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Multiplicity of Aromatic Ring Hydroxylation Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1 Demonstrated by Denaturing Gradient Gel Electrophoresis(Biochemistry & Molecular Biology)
- Complete Degradation of Polychlorinated Biphenyls by a Combination of Ultraviolet and Biological Treatments
- Isolation of Dibenzofuran-Degrading Bacterium, Nocardioides sp. DF412, and Characterization of Its Dibenzofuran Degradation Genes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- 2K10-1 Development of binary vector system for legume transformation based on the soybean-supervirulent Agrobacterium tumefaciens strain KAT23
- Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1
- Diversity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1
- Meta-Fission Product Hydrolases from a Strong PCB Degrader Rhodococcus sp. RHA1
- Nucleotide Sequences and Characterization of Genes Encoding Naphthalene Upper Pathway of Pseudomonas aeruginosa PaK1 and Pseudomonas putida OUS82
- Indigenous Plasmids of Beijerinckia Strains(Microbiology & Fermentation Industry)
- Characterization of polychlorinated biphenyl degradation in a fermentor by Comamonas testosteroni strain TK102
- Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
- Characterization of a Second 2,3-dihydro-2,3-dihydroxybiphenyl Dehydrogenase Gene of the Polychlorinated Biphenyl Degrader Rhodococcus sp. Strain RHA1
- Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
- A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
- Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
- Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
- Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
- Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
- Organogels of Lignin-derived Stable Metabolic Intermediate, 2-Pyrone-4,6-dicarboxylic Acid (PDC), Bearing Cholesteryl Groups
- Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Enhanced Soybean Infection by the Legume "Super-Virulent" Agrobacterium tumefaciens Strain KAT23
- Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
- Preparation of SnS Films by Sulfurization of Sn Sheet
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Enzymatic Properties of Terephthalate 1, 2-Dioxygenase of Comamonas sp. Strain E6
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- Three-dimensional Structure of Microbial 2-Hydroxy1-6-oxo-6-phenylhexa-2, 4-dienoic Acid (HPDA) Hydrolase (BphD Enzyme) from Rhodococcus sp. Strain RHA1, in the PCB Degradation Pathway
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- Successful Treatment of an Esophageal Hemangioma by Endoscopic Injection Sclerotherapy: Report of a Case
- Sulfurization Growth of SnS Films and Fabrication of CdS/SnS Heterojunction for Solar Cells
- Evaluation of Matrix Metalloproteinase-2 (MMP-2) Activity with Film in situ Zymography for Improved Cytological Diagnosis of Breast Tumors
- Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate
- Liquid Crystallinity and Organogelation Behavior of Lignin-Derived Metabolic Intermediate Bearing Cholesterol Groups
- The 24-hp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Photoluminescence Property of ZnSnP2 by Solution Growth and Normal Freezing Methods
- Construction of Broad Host Range Cloning Vectors for Gram-negative Bacteria
- High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates(METHODS)
- Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation
- Characterization of the Third Glutathione S-Transferase Gene Involved in Enantioselective Cleavage of the β-Aryl Ether by Sphingobium sp. Strain SYK-6
- A COMMON ANTIGEN BETWEEN CANDIDA ALBICANS AND MYCOBACTERIUM TUBERCULOSIS
- High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates
- Fatigue Crack Growth Characteristics of Epoxy Resin Reinforced by Silica Particles at Elevated Temperature
- Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Three Cases of Fungemia in HIV-Infected Patients Diagnosed Through the Use of Mycobacterial Blood Culture Bottles
- Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) degradation in Janibacter sp. TYM3221(ENVIRONMENTAL BIOTECHNOLOGY)
- Three-dimensional Structure of 2,3-Dihydroxybiphenyl Dioxygenase (BphC enzyme) from Pseudomonas sp. Strain KKS102 Having Polychlorinated Biphenyl (PCB)-Degrading Activity.
- EXPERIMENTAL STUDIES ON THE TREATMENT OF CANDIDIASIS
- Thermoplastic Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of nitrate and nitrite utilization system in Rhodococcus
- Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.