Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
スポンサーリンク
概要
- 論文の詳細を見る
The vanillin dehydrogenase gene (ligV), which conferred the ability to transform vanillin into vanillate on Escherichia coli, was isolated from Sphingomonas paucimobilis SYK-6. The ligV gene consists of a 1,440-bp open reading frame encoding a polypeptide with a molecular mass of 50,301 Da. The deduced amino acid sequence of ligV showed about 50% identity with the known vanillin dehydrogenases of Pseudomonas vanillin degraders. The gene product of ligV (LigV) produced in E. coli preferred NAD+ to NADP+ and exhibited a broad substrate preference, including vanillin, benzaldehyde, protocatechualdehyde, m-anisaldehyde, and p-hydroxybenzaldehyde, but the activity toward syringaldehyde was less than 5% of that toward vanillin. Insertional inactivation of ligV in SYK-6 indicated that ligV is essential for normal growth on vanillin. On the other hand, growth on syringaldehyde was only slightly affected by ligV disruption, indicating the presence of a syringaldehyde dehydrogenase gene or genes in SYK-6.
- 社団法人 日本農芸化学会の論文
著者
-
Masai Eiji
Nagaoka University of Technology
-
KATAYAMA Yoshihiro
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
-
MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
-
Masai E
Department Of Bioengineering Nagaoka University Of Technology
-
Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
-
Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
-
KASAI Daisuke
Department of Bioengineering, Nagaoka University of Technology
-
YAMAMOTO Yuko
Department of Public Health, School of Medicine, Nihon University
-
INOUE Tomohiko
Department of Bioengineering, Nagaoka University of Technology
-
TAKAMURA Kazuhiro
Department of Bioengineering, Nagaoka University of Technology
-
HARA Hirofumi
Department of Bioengineering, Nagaoka University of Technology
関連論文
- EN-O2 Dibenzofuran degradation system in a Nocardioides sp. DF412(Section VI Environmental Biotechnology)
- Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
- Fusible, elastic, and biodegradable polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC)
- MYB transcription factors orchestrating the developmental program of xylem vessels in Arabidopsis roots
- Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
- Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
- Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
- Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
- Efficiengt Introduction of Vector Plasmids into Acetic Acid Bacteria
- Factors Associated with Exclusive Breast-feeding in Japan : for Activities to Support Child-rearing with Breast-feeding
- EN-P40 Insight into the sugar transport and metabolism in Biphenyl/PCB degrader, Rhodococcus jostii RHA1(Section VI Environmental Biotechnology)
- Enzymatic Properties of Terephthalate 1,2-Dioxygenase of Comamonas sp. Strain E6
- Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
- Identification of a Unique 2,3-Dihydroxybiphenyl 1 ,2- Dioxygenase in Rhodococcus strain RHA1 containing a bphC1 disruption
- Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
- Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
- Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
- Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
- Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
- Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Multiplicity of Aromatic Ring Hydroxylation Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1 Demonstrated by Denaturing Gradient Gel Electrophoresis(Biochemistry & Molecular Biology)
- Complete Degradation of Polychlorinated Biphenyls by a Combination of Ultraviolet and Biological Treatments
- Isolation of Dibenzofuran-Degrading Bacterium, Nocardioides sp. DF412, and Characterization of Its Dibenzofuran Degradation Genes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- 2K10-1 Development of binary vector system for legume transformation based on the soybean-supervirulent Agrobacterium tumefaciens strain KAT23
- Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1
- Diversity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1
- Meta-Fission Product Hydrolases from a Strong PCB Degrader Rhodococcus sp. RHA1
- Nucleotide Sequences and Characterization of Genes Encoding Naphthalene Upper Pathway of Pseudomonas aeruginosa PaK1 and Pseudomonas putida OUS82
- Indigenous Plasmids of Beijerinckia Strains(Microbiology & Fermentation Industry)
- Characterization of polychlorinated biphenyl degradation in a fermentor by Comamonas testosteroni strain TK102
- Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
- Inhibition of P38 MAP Kinase Attenuates Left Ventricular Hypertrophy and Inhibits Progression of Systolic Dysfunction on Pressure-Overload Induced Pathological Cardiac Hypertrophy in Mice
- OE-070 Plasma Soluble Lectin-like Oxidized Low-density Lipoprotein Receptor-1 Level at Admission can Predict In-hospital Prognosis in Patients with Acute Myocardial Infarction(OE12,ACS/AMI (Clinical/Pathophysiology) (IHD),Oral Presentation (English),The 7
- Mutation Analysis ABCC9 Gene in Japanese Patients with Coronary Spastic Angina
- Characterization of a Second 2,3-dihydro-2,3-dihydroxybiphenyl Dehydrogenase Gene of the Polychlorinated Biphenyl Degrader Rhodococcus sp. Strain RHA1
- Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
- A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
- Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
- Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
- Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
- Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
- Specific Accumulation of Polysaccharide-Linked Hydroxycinnamoyl Esters in the Cell Walls of Irregularly Shaped and Collapsed Internode Parenchyma Cells of the Dwarf Rice Mutant Fukei 71
- Immunological detection and cellular localization of the phenylalanine ammonia-lyase of a hybrid aspen
- Isolation and Analysis of Cinnamic Acid 4-Hydroxylase Homologous Genes from a Hybrid Aspen, Populus kitakamiensis
- Organogels of Lignin-derived Stable Metabolic Intermediate, 2-Pyrone-4,6-dicarboxylic Acid (PDC), Bearing Cholesteryl Groups
- Research on cell division control of woody plant
- Enhanced Soybean Infection by the Legume “Super-Virulent” Agrobacterium tumefaciens Strain KAT23
- Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Pericentric inversion inv (2) (p11.2q21) associated with Treacher Collins-Franceschetti syndrome
- Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
- Enhanced Soybean Infection by the Legume "Super-Virulent" Agrobacterium tumefaciens Strain KAT23
- Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
- Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
- Enzymatic Properties of Terephthalate 1, 2-Dioxygenase of Comamonas sp. Strain E6
- Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
- Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
- Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
- Three-dimensional Structure of Microbial 2-Hydroxy1-6-oxo-6-phenylhexa-2, 4-dienoic Acid (HPDA) Hydrolase (BphD Enzyme) from Rhodococcus sp. Strain RHA1, in the PCB Degradation Pathway
- Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
- PJ-313 Cardiac Resynchronization Therapy Programming Using Impedance Cardiography(Heart failure, clinical(15)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate
- Liquid Crystallinity and Organogelation Behavior of Lignin-Derived Metabolic Intermediate Bearing Cholesterol Groups
- The 24-hp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation
- Characterization of the Third Glutathione S-Transferase Gene Involved in Enantioselective Cleavage of the β-Aryl Ether by Sphingobium sp. Strain SYK-6
- Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) degradation in Janibacter sp. TYM3221(ENVIRONMENTAL BIOTECHNOLOGY)
- Thermoplastic Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
- Characterization of nitrate and nitrite utilization system in Rhodococcus