Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
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概要
- 論文の詳細を見る
The vanillin dehydrogenase gene (ligV), which conferred the ability to transform vanillin into vanillate on Escherichia coli, was isolated from Sphingomonas paucimobilis SYK-6. The ligV gene consists of a 1,440-bp open reading frame encoding a polypeptide with a molecular mass of 50,301 Da. The deduced amino acid sequence of ligV showed about 50% identity with the known vanillin dehydrogenases of Pseudomonas vanillin degraders. The gene product of ligV (LigV) produced in E. coli preferred NAD+ to NADP+ and exhibited a broad substrate preference, including vanillin, benzaldehyde, protocatechualdehyde, m-anisaldehyde, and p-hydroxybenzaldehyde, but the activity toward syringaldehyde was less than 5% of that toward vanillin. Insertional inactivation of ligV in SYK-6 indicated that ligV is essential for normal growth on vanillin. On the other hand, growth on syringaldehyde was only slightly affected by ligV disruption, indicating the presence of a syringaldehyde dehydrogenase gene or genes in SYK-6.
- 社団法人 日本農芸化学会の論文
- 2007-10-23
著者
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Fukuda Masao
Nagaoka University of Technology
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Kasai Daisuke
Nagaoka University of Technology
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Masai Eiji
Nagaoka University of Technology
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KATAYAMA Yoshihiro
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
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Masai E
Department Of Bioengineering Nagaoka University Of Technology
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Masai Eiji
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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Kasai Daisuke
Nagaoka University Of Technology Department Of Bioengineering
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Fukuda Masao
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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KASAI Daisuke
Department of Bioengineering, Nagaoka University of Technology
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YAMAMOTO Yuko
Department of Public Health, School of Medicine, Nihon University
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Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Yamashita Kenji
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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INOUE Tomohiko
Department of Bioengineering, Nagaoka University of Technology
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TAKAMURA Kazuhiro
Department of Bioengineering, Nagaoka University of Technology
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HARA Hirofumi
Department of Bioengineering, Nagaoka University of Technology
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Yajima Kazuyoshi
Sano College Sano Nihon University Gakuen
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Katayama Yoshihiro
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Yamada K
Faculty Of Engineering Okayama University:(present Address)department Of Biotechnology And Life Scie
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Yamamoto Yuko
Department Of Cardiology Hirosaki University Graduate School Of Medicine Hirosaki Japan
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Katayama Y
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Hara Hirofumi
Department Of Bioengineering Nagaoka University Of Technology
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Inoue Tomohiko
Department Of Bioengineering Nagaoka University Of Technology
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Katayama Yoshihiro
Graduate School Of Bio-applications & System Engineering Tokyo University Of Agriculture And Tec
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Takamura Kazuhiro
Department Of Bioengineering Nagaoka University Of Technology
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Kasai Daisuke
Department Of Bioengineering Nagaoka University Of Technology
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Yamamoto Yuko
Department Of Bioengineering Nagaoka University Of Technology
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Katayama Yoshihiro
College of Bioresource Sciences, Nihon University
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