Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1

スポンサーリンク

概要

• 論文の詳細を見る

• Rhodococcus sp. RHA1 induces two biphenyl dioxygenases, the BphA and EtbA/EbdA dioxygenases, during growth on biphenyl. Their subunit genes were expressed in R. erythropolis IAM1399 to investigate the involvement of each subunit gene in their activity and their substrate preferences. The recombinant expressing ebdA1A2A3etbA4 and that expressing bphA1A2A3A4 exhibited 4-chlorobiphenyl (4-CB) transformation activity, suggesting that these gene sets are responsible for the EtbA/EbdA and BphA dioxygenases respectively. When bphA4 and etbA4 were swapped to construct the recombinants expressing ebdA1A2A3bphA4 and bphA1A2A3etbA4 respectively, compatibility between BphA4 and EtbA4 was suggested by their 4-CB transformation activities. When bphA3 and ebdA3 were swapped, incompatibility between BphA3 and EbdA3 was suggested. BphA and EtbA/EbdA dioxygenases exhibited the highest transformation activity toward biphenyl and naphthalene respectively, and also attacked dibenzofuran and dibenzo-p-dioxin. The wide substrate preference of EtbA/EbdA dioxygenase suggested that it plays a more important role in polychlorinated biphenyl (PCB) degradation than does BphA dioxygenase.

• 社団法人 日本農芸化学会の論文
• 2007-04-23

著者

• Fukuda Masao

  Nagaoka University of Technology

• Masai Eiji

  Nagaoka University of Technology

• MASAI Eiji

  Department of Bioengineering, Nagaoka University of Technology

• Masai E

  Department Of Bioengineering Nagaoka University Of Technology

• Masai Eiji

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• Masai Eiji

  Department Of Bioengineering Nagaoka University Of Engineering

• Fukuda Masao

  Department Of Bioengineering Nagaoka University Of Technology

• Fukuda Masao

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• TAKEDA Hisashi

  Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine

• MIYAUCHI Keisuke

  Department of Civil and Environmental Engineering, Tohoku Gakuin University

• Fukuda Masao

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Takeda Hisashi

  Department Of Biophysical Genetics Kanazawa University Graduate School Of Medicine

• Miyauchi Keisuke

  Department Of Bioengineering Nagaoka University Of Technology

• IWASAKI Takumi

  Department of Bioengineering, Nagaoka University of Technology

• YAMADA Tadakazu

  Sugiyama Chemical and Industrial Laboratory

• Miyauchi Keisuke

  Department Of Civil And Environmental Engineering Tohoku Gakuin University

• Tanaka Hiroshi

  National Institute Of Agrobiological Sciences

• Iwasaki Takumi

  Department Of Bioengineering Nagaoka University Of Technology

• Takeda Hisashi

  National Institute of Agrobiological Sciences

関連論文

• EN-O2 Dibenzofuran degradation system in a Nocardioides sp. DF412(Section VI Environmental Biotechnology)
• Mechanical Properties of Poly(L-Lactide) Films Controlled by Blending with Polyesters of Lignin-Derived Stable Metabolic Intermediate, 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
• Polyesters of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as Bio-based Plastics Exhibiting Strong Adhering Properties
• Fusible, elastic, and biodegradable polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC)
• Overexpression of human CD38/ADP-ribosyl cyclase enhances acetylcholine-induced Ca^ signalling in rodent NG108-15 neuroblastoma cells
• Modification of Rice Plant Height by Suppressing the Height-Controlling Gene, D18, in Rice
• Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1
• Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
• Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
• Fusible, Elastic, and Biodegradable Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC)
• Nucleotide Sequence of the Gene Encoding the 45-Kilodalton Subunit of Alcohol Dehydrogenase from Acetobacter aceti
• Possible Functional Domains in a Quinoprotein Alcohol Dehydrogenase from Acetobacter aceti
• Efficiengt Introduction of Vector Plasmids into Acetic Acid Bacteria
• CHARACTERIZATION OF GIBBERELLIN 2β-HYDROXYLASE GENE FROM RICE (ORYZA SATIVAL.)
• EN-P40 Insight into the sugar transport and metabolism in Biphenyl/PCB degrader, Rhodococcus jostii RHA1(Section VI Environmental Biotechnology)
• Enzymatic Properties of Terephthalate 1,2-Dioxygenase of Comamonas sp. Strain E6
• Identification of Insertion Sequence from a γ-Hexachlorocyclohexane Degrading Bacterium, Sphingomonas paucimobilis UT26
• Identification of a Unique 2,3-Dihydroxybiphenyl 1 ,2- Dioxygenase in Rhodococcus strain RHA1 containing a bphC1 disruption
• Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis
• Isolation and Characterization of Tn5-Induced Mutants of Pseudomonas paucimobilis UT26 Defective in γ-Hexachlorocyclohexane Dehydrochlorinase (LinA)
• Degradation of Ethylbenzene by Pseudomonas putida Harboring OCT Plasmid(Biological Chemistry)
• Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis(Microbiology & Fermentation Industry)
• Isolation and Characterization of a Mixed Culture That Degrades Polychlorinated Biphenyls(Microbiology & Fermentation Industry)
• Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6
• Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
• Multiplicity of Aromatic Ring Hydroxylation Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1 Demonstrated by Denaturing Gradient Gel Electrophoresis(Biochemistry & Molecular Biology)
• Complete Degradation of Polychlorinated Biphenyls by a Combination of Ultraviolet and Biological Treatments
• Isolation of Dibenzofuran-Degrading Bacterium, Nocardioides sp. DF412, and Characterization of Its Dibenzofuran Degradation Genes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
• 2K10-1 Development of binary vector system for legume transformation based on the soybean-supervirulent Agrobacterium tumefaciens strain KAT23
• Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1
• Diversity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in a Strong PCB Degrader, Rhodococcus sp. Strain RHA1
• Meta-Fission Product Hydrolases from a Strong PCB Degrader Rhodococcus sp. RHA1
• Nucleotide Sequences and Characterization of Genes Encoding Naphthalene Upper Pathway of Pseudomonas aeruginosa PaK1 and Pseudomonas putida OUS82
• Indigenous Plasmids of Beijerinckia Strains(Microbiology & Fermentation Industry)
• Characterization of polychlorinated biphenyl degradation in a fermentor by Comamonas testosteroni strain TK102
• Molecular Properties of 2-Pyrone-4,6-dicarboxylic Acid (PDC) as a Stable Metabolic Intermediate of Lignin Isolated by Fractional Precipitation with Na^+ Ion
• Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
• Function, Intracellular Localization and the Importance in Salt Tolerance of a Vacuolar Na^+/H^+ Antiporter from Rice
• THE FUNCTIONAL ANALYSIS OF THE RICE Na^+/H^+ ANTIPORTER GENE
• Characterization of a Second 2,3-dihydro-2,3-dihydroxybiphenyl Dehydrogenase Gene of the Polychlorinated Biphenyl Degrader Rhodococcus sp. Strain RHA1
• Tenacious Epoxy Adhesives Prepared from Lignin-derived Stable Metabolic Intermediate
• A Novel Biomass-based Polymer Prepared from Lignin-derived Stable Metabolic Intermediate by Copper(I)-catalyzed Azide-Alkyne Click Reaction
• Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
• Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
• Tetrahydrofolate-dependent vanillate and syringate O-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6
• Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenative polymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli
• Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6 : interaction of O-demethylase(LigX)and ring fission dioxygenase(LigZ)
• Characterization of the Cα-Dehydrogenase Gene Involved in the Cleavage of β-Aryl Ether by Pseudomonas paucimobilis
• Organogels of Lignin-derived Stable Metabolic Intermediate, 2-Pyrone-4,6-dicarboxylic Acid (PDC), Bearing Cholesteryl Groups
• Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation
• Enhanced Soybean Infection by the Legume “Super-Virulent” Agrobacterium tumefaciens Strain KAT23
• Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
• Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
• Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
• In vitro but not in planta encapsidation of Rice gall dwarf virus core particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants
• Detection of bphAa Gene Expression of Rhodococcus sp. Strain RHA1 in Soil Using a New Method of RNA Preparation from Soil
• A New Method to Calculate the Green Function by Polynomial Expansion
• Enhanced Soybean Infection by the Legume "Super-Virulent" Agrobacterium tumefaciens Strain KAT23
• Characterization and Host Range Determination of Soybean Super Virulent Agrobacterium tumefaciens KAT23
• Preparation of SnS Films by Sulfurization of Sn Sheet
• Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412
• Enzymatic Properties of Terephthalate 1, 2-Dioxygenase of Comamonas sp. Strain E6
• Characterization of Two Biphenyl Dioxygenases for Biphenyl/PCB Degradation in A PCB Degrader, Rhodococcus sp. Strain RHA1
• Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
• Characterization of ligV Essential for Catabolism of Vanillin by Sphingomonas paucimobilis SYK-6
• Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
• Three-dimensional Structure of Microbial 2-Hydroxy1-6-oxo-6-phenylhexa-2, 4-dienoic Acid (HPDA) Hydrolase (BphD Enzyme) from Rhodococcus sp. Strain RHA1, in the PCB Degradation Pathway
• Expression in Escherichia coli of Biphenyl 2,3-Dioxygenase Genes from a Gram-Positive Polychlorinated Biphenyl Degrader, Rhodococcus jostii RHA1
• Successful Treatment of an Esophageal Hemangioma by Endoscopic Injection Sclerotherapy: Report of a Case
• Sulfurization Growth of SnS Films and Fabrication of CdS/SnS Heterojunction for Solar Cells
• Evaluation of Matrix Metalloproteinase-2 (MMP-2) Activity with Film in situ Zymography for Improved Cytological Diagnosis of Breast Tumors
• Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate
• Liquid Crystallinity and Organogelation Behavior of Lignin-Derived Metabolic Intermediate Bearing Cholesterol Groups
• The 24-hp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Photoluminescence Property of ZnSnP2 by Solution Growth and Normal Freezing Methods
• Construction of Broad Host Range Cloning Vectors for Gram-negative Bacteria
• High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates(METHODS)
• Relationship between macular ganglion cell complex parameters and visual field parameters after tumor resection in chiasmal compression
• Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation
• Characterization of the Third Glutathione S-Transferase Gene Involved in Enantioselective Cleavage of the β-Aryl Ether by Sphingobium sp. Strain SYK-6
• A COMMON ANTIGEN BETWEEN CANDIDA ALBICANS AND MYCOBACTERIUM TUBERCULOSIS
• High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates
• Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) degradation in Janibacter sp. TYM3221(ENVIRONMENTAL BIOTECHNOLOGY)
• Three-dimensional Structure of 2,3-Dihydroxybiphenyl Dioxygenase (BphC enzyme) from Pseudomonas sp. Strain KKS102 Having Polychlorinated Biphenyl (PCB)-Degrading Activity.
• EXPERIMENTAL STUDIES ON THE TREATMENT OF CANDIDIASIS
• Thermoplastic Polyesters of 2-Pyrone-4,6-Dicarboxylic Acid (PDC) Obtained from a Metabolic Intermediate of Lignin
• Relationship between optic disc ovality and horizontal disc tilt in normal young subjects
• Differentiation by imaging of superior segmental optic hypoplasia and normal-tension glaucoma with inferior visual field defects only
• Characterization of nitrate and nitrite utilization system in Rhodococcus
• Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.

もっと見る 閉じる

スポンサーリンク