Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
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Rhodococcus jostii RHA1 accumulates chlorobenzoates (CBA) during the degradation of polychlorinated biphenyls (PCBs). CBA degradation is considered one of the rate-limiting steps in the complete degradation of PCBs. To reduce the accumulation of CBAs, the upper pathway enzyme genes for PCB degradation of RHA1 were introduced into a CBA-degrading bacterium, Burkholderia sp. NK8. The resulting recombinant strain exhibited no biphenyl 2,3-dioxygenase (BphA) activity encoded by bphAaAbAcAd genes, which encode the large and small subunits of the terminal oxygenase component and the ferredoxin and reductase subunits responsible for electron transfer from NADH to the large subunit. The remaining enzyme genes involved in the transformation of biphenyl to benzoate, bphB2C1D1, which encode dehydrogenase, ring-cleavage dioxygenase and hydrolase, conferred activities to NK8. To obtain the BphA activity of RHA1 in NK8, sets of BphA genes were constructed by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, encoding the ferredoxin and reductase subunits. Hybrid derivatives of BphA containing the KF707 bphA3 conferred BphA activity to NK8, and a derivative containing the RHA1 bphAaAb and KF707 bphA3A4 genes exhibited the highest BphA activity. A plasmid containing the RHA1 bphAaAb and KF707 bphA3A4 genes plus the RHA1 bphB2C1D1 genes was constructed and introduced into NK8. The resulting recombinant strain efficiently degraded 2-, 3- and 4-chlorobiphenyls with an apparent reduction in CBA accumulation in comparison to the recombinant mutant strain, which had an insertion in the cbeA gene to inactivate CBA dioxygenase.
著者
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Fukuda Masao
Nagaoka University of Technology
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Kasai Daisuke
Nagaoka University of Technology
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Masai Eiji
Nagaoka University of Technology
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MASAI Eiji
Department of Bioengineering, Nagaoka University of Technology
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Masai E
Department Of Bioengineering Nagaoka University Of Technology
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Masai Eiji
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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Masai Eiji
Department Of Bioengineering Nagaoka University Of Engineering
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Fukuda Masao
Department Of Bioengineering Nagaoka University Of Technology
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Kasai Daisuke
Nagaoka University Of Technology Department Of Bioengineering
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Fukuda Masao
Dep. Of Bioengineering Nagaoka Univ. Of Technol.
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Furukawa Kensuke
Department of Agricultural Chemistry, Kyushu University
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Ohmori Tsuneo
Department of Bioengineering, Nagaoka University of Technology
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Morita Hirokazu
Department of Bioengineering, Nagaoka University of Technology
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Tanaka Megumi
Department of Bioengineering, Nagaoka University of Technology
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MIYAUCHI Keisuke
Department of Civil and Environmental Engineering, Tohoku Gakuin University
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KASAI Daisuke
Department of Bioengineering, Nagaoka University of Technology
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Fukuda Masao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Furukawa Kensuke
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Miyauchi Keisuke
Department Of Bioengineering Nagaoka University Of Technology
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OGAWA Naoto
Faculty of Agriculture, Kyushu University
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Ogawa Naoto
Faculty Of Agriculture Kyushu University
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MIYASHITA Kiyotaka
National Institute for Agro-Environmental Sciences
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Miyauchi Keisuke
Department Of Civil And Environmental Engineering Tohoku Gakuin University
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Morita Hirokazu
Department Of Bioengineering Nagaoka University Of Technology
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Furukawa Kensuke
Department Of Food And Bioscience Faculty Of Food Science And Nutrition Beppu University
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Miyashita Kiyotaka
National Inst. For Agro-environmental Sciences
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Ohmori Tsuneo
Department Of Bioengineering Nagaoka University Of Technology
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Morita Hirokazu
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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Tanaka Megumi
Department Of Bioengineering Nagaoka University Of Technology
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Kasai Daisuke
Department Of Bioengineering Nagaoka University Of Technology
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