Engineering a Hybrid Pseudomonad to Acquire 3, 4-Dioxygenase Activity for Polychlorinated Biphenyls

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概要

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• We constructed a hybrid strain that acquired 3, 4-dioxygenase activity for polychlorinated biphenyls(PCBs). This strain, KF707-D34, possessed a chimeric biphenyl dioxygenase gene, of which a portion of bphA(coding for a large subunit of biphenyl dioxygenase)of Pseudomonas pseudoalcaligenes KF707 was replaced with that of a PCB-degrader, Burkholderia cepacia LB400 by homologous recombination. KF707-D34 retained the ability to degrade 4, 4'-dichlorobiphenyl via 2, 3-dioxygenation in a fashion identical to that of KF707 and gained novel capability to degrade 2, 5, 4'-trichlorobiphenyl and 2, 5, 2', 5'-tetrachlorobiphenyl via 3, 4-dioxygenation in a fashion identical to that of LB400. Sequence analysis of bphA1 from KF707-D34 revealed that three nucleotides in the 3'-terminal region of KF707 bphA1 were changed to correspond to those in LB400 bphA1. The resulting BphA1 protein in KF707-D34 was changed at position 376 from threonine(Thr)to asparagine(Asn). The results demonstrate that a minor alteration of the amino acid sequence in BphA1 improved the PCB degradation capability in biphenyl-utilizing bacteria.

• 社団法人日本生物工学会の論文
• 1999-04-25

著者

• Sakai Masashi

  Department Of Cardiovascular Science And Medicine Chiba University Graduate School Of Medicine

• Furukawa Kensuke

  Department of Agricultural Chemistry, Kyushu University

• Suenaga Hikaru

  Department Of Agricultural Chemistry Kyushu University

• Furukawa Kensuke

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• NISHI AKITO

  Department of Agricultural Chemistry, Kyushu University

• Nishi Akito

  Department Of Agricultural Chemistry Kyushu University

• WATANABE TAKAHITO

  Department of Agricultural Chemistry, Kyushu University

• Watanabe Takahito

  Department Of Agricultural Chemistry Kyushu University

• Sakai Masashi

  Department Of Biology Washington University

• SAKAI Masashi

  Department of Applied Chemistry and Biochemistry, Kyushu Sangyo University

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